Long non-coding RNA PVT1-5 promotes cell proliferation by regulating miR-126/SLC7A5 axis in lung cancer

Journal Article · Mon Jan 15 04:00:00 EST 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2017.12.114· OSTI ID:23127456

Li, Hongliang ^[1]; Chen, Shuxian ^[2]; Liu, Jia ^[3]; Guo, Xiaopeng ^[1]; Xiang, Xudong ^[4]; others, and

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1. School of Medicine, Yunnan University, Kunming (China)
2. Department of Hepatobiliary Surgery, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou (China)
3. Department of Pharmacy, Kunming Medical University Haiyuan College, Kunming (China)
4. Department of Thoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming (China)

Highlights: • LncRNA-PVT1-5 is up-regulated in lung cancer tissues and cell lines. • LncRNA-PVT1-5 acted as a sponge for miR-126 to regulate cells proliferation in lung cancer cells. • LncRNA-PVT1-5 exerts its role via miR-126/SLC7A5 axis in lung cancer cell modes. Dysregulated long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) play key roles in the development of human cancers. The lncRNA plasmacytoma variant translocation 1 (PVT1) is reported to be an oncogene in a variety of cancers. However, the roles of PVT1-5 and its related miRNAs in lung cancer are poorly understood. In this study, we found that PVT1-5 expression was significantly increased in lung cancer tissues and cell lines. By using biotin-labeled lncRNA-PVT1-5 probe for miRNA in vivo precipitation (miRIP) in lung cancer cells and dual-luciferase reporterassays, we identified that miR-126 was associated with lncRNA-PVT1-5. Furthermore, knockdown of lncRNA-PVT1-5 in cells could down-regulate the expression of SLC7A5, the target of oncogenic miR-126, resulting in the cell proliferation. Conversely, inhibiting the expression of miR-126 markedly increased the expression of SLC7A5 and alleviated cell proliferation inhibition. Thus, our results indicated that lncRNA-PVT1-5 may function as a competing endogenous RNA (ceRNA) for miR-126 to promote cell proliferation by regulating the miR-126/SLC7A5 pathway, suggesting that lncRNA-PVT1-5 plays a crucial role in lung cancer progression and lncRNA-PVT1-5/miR-126/SLC7A5 regulatory network may shed light on tumorigenesis in lung cancer.

• OSTI ID: 23127456
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 495; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BIOTIN
CELL PROLIFERATION
LUCIFERASE
LUNGS
PORIFERA
RNA