Anticancer activity of arborinine from Glycosmis parva leaf extract in human cervical cancer cells
Journal Article
·
· Biochemical and Biophysical Research Communications
- National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency, Pathum Thani (Thailand)
- Department of Pharmacognosy, Faculty of Pharmacy, Rangsit University, Pathum Thani (Thailand)
- Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok (Thailand)
Highlights: • Arborinine extracted from Glycosmis parva leaves has strong anticancer activity. • It is significantly more cytotoxic to HeLa cancer cells than normal cells. • Arborinine inhibits tumor spheroid growth more potently than chemotherapeutic drugs cisplatin, gemcitabine, and bleomycin. • Arborinine induces DNA damage-independent apoptosis. • It inhibits cancer cell migration by downregulating epithelial-mesenchymal transition. Glycosmis parva is a small shrub found in Thailand. Ethyl acetate (EtOAc) extract from its leaves has been shown to exert anticancer effects in vitro; however, the compound responsible for this activity has not been isolated and characterized. In this study, we demonstrate that arborinine, a major acridone alkaloid in the EtOAc fraction, decreased proliferation and was strongly cytotoxic to HeLa cervical cancer cells without significantly affecting normal cells. The compound also inhibited tumor spheroid growth much more potently than chemotherapeutic drugs bleomycin, gemcitabine, and cisplatin. In addition, unlike cisplatin, arborinine activated caspase-dependent apoptosis without inducing DNA damage response. We further show that arborinine strongly suppressed cancer cell migration by downregulating expression of key regulators of epithelial-mesenchymal transition. Taken together, our data provide important insights into the molecular mechanism of arborinine’s anticancer activity, supporting its potential use for treating cervical cancer.
- OSTI ID:
- 23125151
- Journal Information:
- Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 500; ISSN 0006-291X; ISSN BBRCA9
- Country of Publication:
- United States
- Language:
- English
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