MiR-130a-3p inhibits migration and invasion by regulating RAB5B in human breast cancer stem cell-like cells

Journal Article · Fri Jun 15 04:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.05.018· OSTI ID:23125115

Kong, Xiangjie ^[1]; Zhang, Junfeng; Li, Jia ^[2]; Shao, Jianfeng ^[1]; Fang, Lin ^[2]

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1. Department of Urology, Wuxi People's Hospital, Nanjing Medical University, Wuxi, Jiangsu (China)
2. Department of Breast and Thyroid, Shanghai Tenth People's Hospital, Tongji University, Shanghai (China)

Highlights: • We enriched breast cancer stem-like cells expressing CD44{sup +}CD24{sup -/low} from MCF-7 cells. • We found that miR-130a-3p is down-regulated in human breast cancer tissues and exosomes from circulating blood, and overexpression of miR-130a-3p in BCSCs inhibited cellular proliferation, migration, and invasion. • MiR-130a-3p inhibits migration and invasion by regulating RAB5B in human breast cancer stem cell-like cells. Breast cancer stem cells (BCSCs) constitute a subpopulation of tumor cells that express stem cell-associated markers and have a high capacity for tumor generation in vivo. MicroRNAs (miRNAs) are involved in tumorigenesis by regulating specific oncogenes and tumor suppressor genes, and their roles in BCSCs are becoming more apparent. We try to reveal the mechanism by which specific miRNA plays its function in BCSCs. Herein, we show that miR-130a-3p is down-regulated in human breast cancer tissues and exosomes from circulating blood. Overexpression of miR-130a-3p in BCSCs inhibited cellular proliferation, migration, and invasion, and silencing of miR-130a-3p had the opposite effects. We also confirmed that RAB5B is directly down-regulated by miR-130a-3p. Knockdown of RAB5B also inhibited cell proliferation, migration and invasion. Furthermore, we found that lower levels of exosome-derived miR-130a-3p are associated with lymph node metastasis and advanced TNM stage. Taken together, our results demonstrate that miR-130a-3p may act as a disease progression monitoring indicator and therapeutic target in breast cancer.

• OSTI ID: 23125115
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 501; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BLOOD
CELL PROLIFERATION
MAMMARY GLANDS
STEM CELLS
TUMOR CELLS