Short- and long-term effects of high-fat diet feeding and voluntary exercise on hepatic lipid metabolism in mice

Journal Article · Sat Dec 15 04:00:00 EST 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.11.026· OSTI ID:23107889

Yoshimura, Saki ^[1]; Nakashima, Shihoko ^[2]; Tomiga, Yuki ^[3]; Kawakami, Shotaro ^[4]; Uehara, Yoshinari; Higaki, Yasuki ^[2]

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1. Graduate School of Sports and Health Science, Fukuoka University, 8-19-1 Nanakuma, Johnan-ku, Fukuoka, 814-0180 (Japan)
2. Faculty of Health and Sports Science, Fukuoka University, 8-19-1 Nanakuma, Johnan-ku, Fukuoka, 814-0180 (Japan)
3. The Fukuoka University Institute for Physical Activity, 8-19-1 Nanakuma, Johnan-ku, Fukuoka, 814-0180 (Japan)
4. Department of Rehabilitation, Fukuoka University Chikushi Hospital, 1-1-1 Zokumyoin, Chikushino, Fukuoka, 818-0067 (Japan)

Highlights: • Voluntary exercise for 1 week (short-term exercise) significantly reduced fat accumulation in the liver. • Short-term exercise induced a downregulation of lipogenic proteins and an upregulation of lipolytic proteins in liver. • Long-term voluntary exercise downregulated the expression of hepatic lipogenesis-associated proteins. Exercise is an effective tool for improving high-fat diet induced fat accumulation in the liver. However, the process of fat accumulation in the liver and the efficacy of early intervention with exercise remain unclear. The aim of this study was to investigate the short- and long-term effects of high-fat diet feeding and voluntary exercise on hepatic lipid metabolism in mice. Male C57BL/6J mice aged 6 weeks were randomly divided into two groups, the control group and high-fat diet feeding group, and fed a normal or high-fat diet for 12 weeks. After 6 weeks, mice in the high-fat diet feeding group were further divided into no exercise group and voluntary exercise training group, with mice in the exercise group provided a running wheel for 6 weeks. Body weight, food intake, and wheel rotation counts were measured every second day for 12 weeks. We found that voluntary exercise for 1 week (short-term exercise) significantly reduced fat accumulation in the liver by downregulating the expression of hepatic lipogenesis-associated proteins and upregulating the expression of hepatic lipolysis-associated proteins, as determined through western blotting and histology. Further, voluntary exercise for 6 weeks (long-term exercise) downregulated the expression of hepatic lipogenesis-associated proteins. These results suggest that hepatic lipogenesis and/or hepatic lipolysis mediate the beneficial effects of voluntary exercise on hepatic fat accumulation.

• OSTI ID: 23107889
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1-4 Vol. 507; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
DIET
EXERCISE
FATS
LIVER
METABOLISM
MICE
PROTEINS