Evaluation of collagen mixture on promoting skin wound healing in zebrafish caused by acetic acid administration
- Yabao Pharmaceutical Group Co., Ltd, Fenglingdu, Shanxi, 044602 (China)
- The Center for Disease Control and Prevention of Shaanxi Province, Xi'an, Shaanxi, 710054 (China)
- Zhejiang Chinese Medical University, Hangzhou (China)
- Hunter Biotechnology, Inc, Hangzhou (China)
- Department of Chemistry, Chinese Culture University, Taipei, 11114 (China)
- Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li, 32023 (China)
The aim of this study is to use zebrafish embryos as a quick platform for wound healing studies. At beginning, we optimized a protocol to induce skin lesion by acetic acid injection. The acetic acid injection induced regional inflammation wound hyperpigmentation by recruiting pigment cells to the wound area. Later, we applied established platform to evaluate the effect of tilapia's collagen peptide mixtures, including demonstration on promoting skin wound healing and eliminating inflammatory response. Results showed that after treating TY001, one of the above fish collagen peptide mixtures, not only repair and proliferation were induced, but also death and apoptosis cells were cleared within cutaneous lesion. Moreover, inflammatory response was suppressed along with collagen mixture treatment. Finally, the TY001-associated signaling was validated by real time-PCR, and numbers of gene associated with tissue repair and vessel proliferation were induced. To sum up, our findings provided a permissive model that may apply to generate a platform for further screening on repair and restoration technology. In addition, the tilapia fish collagen peptide mixture we applied on our model has great potential on developing clinical application on wound healing.
- OSTI ID:
- 23107796
- Journal Information:
- Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 505; ISSN 0006-291X; ISSN BBRCA9
- Country of Publication:
- United States
- Language:
- English
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