The long noncoding RNA XIST protects cardiomyocyte hypertrophy by targeting miR-330-3p

Journal Article · Thu Nov 15 04:00:00 EST 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.09.135· OSTI ID:23107768

Chen, Yuewu; Liu, Xianxia; Chen, Lei; Chen, Weiwei; Zhang, Yuansheng; Chen, Jiaxian; Wu, Xuezheng; Zhao, Yong; Wu, Xiaoyan; Sun, Guowei ^[1]

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1. Department of Cardiovascular Medicine, The Second Affiliated Hospital of Hainan Medical University, Haikou, Hainan Province, 570100 (China)

Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are implicated in numerous kinds of cardiovascular diseases, and their vital role in regulating cardiac hypertrophy still needs to be explored. In this study, we demonstrated that lncRNA X-inactive specific transcript (XIST) was upregulated in hypertrophic cardiac of mice and phenylephrine (PE)-treated cardiomyocytes. Next, we observed that inhibition of XIST induced hypertrophic response of cardiomyocyte and overexpression of XIST attenuated cardiomyocyte hypertrophy induced by PE. Furthermore, through online predictive tools and functional experiments, we demonstrated that XIST and S100B were targets of miR-330-3p. XIST and miR-330-3p suppressed each other in a reciprocal way in cardiomyocytes. Additionally, XIST promoted S100B expression through harboring the complementary binding sites with miR-330-3p, eventually prevented cardiac hypertrophy. In conclusion, our findings revealed a novel molecular mechanism that XIST/miR-330-3p/S100B pathway modulates the progression of cardiomyocyte hypertrophy.

• OSTI ID: 23107768
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 505; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CARDIOVASCULAR DISEASES
HYPERTROPHY
MICE
RNA