Regeneration of the oesophageal muscle layer from oesophagus acellular matrix scaffold using adipose-derived stem cells
- Laboratory for Stem Cell Research, Aalborg University, Fredrik Bajers Vej 3B, 9220, Aalborg (Denmark)
- Sir Alan Parks Physiology Unit, St. Mark's Hospital, Northwick Park, Watford Road, Harrow, HA1 3UJ (United Kingdom)
- Northwick Park Institute for Medical Research (NPIMR), Block Y, Level 3, Northwick Park, Watford Road, Harrow, HA1 3UJ (United Kingdom)
Highlights: • Production of acellular matrix was achieved with complete removal of nuclear material but retaining collagen. • Human adipose-derived stem cells could attach and adhere to decellularized oesophageal acellular matrix. • Cells survived and proliferated for at least 7 days and oxygen level did not affect the proliferative capacity. This study explored the feasibility of constructing a tissue engineered muscle layer in the oesophagus using oesophageal acellular matrix (OAM) scaffolds and human aortic smooth muscle cells (hASMCs) or human adipose-derived stem cells (hASCs). The second objective was to investigate the effect of hypoxic preconditioning of seeding cells on cell viability and migration depth. Our results demonstrated that hASMCs and hASCs could attach and adhere to the decellularized OAM scaffold and survive and proliferate for at least 7 days depending on the growth conditions. This indicates adipose-derived stem cells (ASCs) have the potential to substitute for smooth muscle cells (SMCs) in the construction of tissue engineered oesophageal muscle layers.
- OSTI ID:
- 23105703
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 503, Issue 1; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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