Crystal structure of Escherichia coli DEAH/RHA helicase HrpB

Journal Article · Sat Sep 15 04:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.08.191· OSTI ID:23103533

Xin, Ben-Ge; Chen, Wei-Fei ^[1]; Rety, Stephane ^[2]; Dai, Yang-Xue ^[1]; Xi, Xu-Guang ^[1]

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1. College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100 (China)
2. Univ. Lyon, ENS de Lyon, Univ. Claude Bernard, CNRS UMR 5239, INSERM U1210, LBMC, 46 allée d'Italie Site Jacques Monod, F-69007, Lyon (France)

Highlights: • Structure of Escherichia coli HrpB in a complex with ADP• AlF{sub 4} is reported. • The CTE domain of EcHrpB is unique and harbors eight helices and nine β-sheets. • EcHrpB may function in helicase activity-independent RNA metabolic processes. RNA helicases are almost ubiquitous important enzymes that take part in multiple aspects of RNA metabolism. Prokaryotes encode fewer RNA helicases than eukaryotes, suggesting that individual prokaryotic RNA helicases may take on multiple roles. The specific functions and molecular mechanisms of bacterial DEAH/RHA helicases are poorly understood, and no structures are available of these bacterial enzymes. Here, we report the first crystal structure of the DEAH/RHA helicase HrpB of Escherichia coli in a complex with ADP• AlF{sub 4}. It showed an atypical globular structure, consisting of two RecA domains, an HA2 domain and an OB domain, similar to eukaryotic DEAH/RHA helicases. Notably, it showed a unique C-terminal extension that has never been reported before. Activity assays indicated that EcHrpB binds RNA but not DNA, and does not exhibit unwinding activity in vitro. Thus, within cells, the EcHrpB may function in helicase activity-independent RNA metabolic processes.

• OSTI ID: 23103533
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 504; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADP
CRYSTAL STRUCTURE
DNA
ENZYMES
ESCHERICHIA COLI
METABOLISM
RNA