Wound-induced polypeptides improve resistance against Pseudomonas syringae pv. tomato DC3000 in Arabidopsis

Journal Article · Sat Sep 15 00:00:00 EDT 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.08.147· OSTI ID:23103528

Yu, Liangliang; Wang, Yawen; Liu, Yan; Li, Ningning; Yan, Junhui; Luo, Li ^[1]

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1. Shanghai Key Lab of Bio-energy Crops, Plant Science Center, School of Life Sciences, Shanghai University, Shanghai, 200444 (China)

Wound-induced polypeptides (WIPs) are a novel class of polypeptides with the length less than 100 amino acids. Our previous research has identified a number of WIP genes in soybean (Glycine max) root nodules. However, functions of WIPs in planta remains largely unknown. Here, we identified five WIP-encoding genes, AtWIP1-5, in Arabidopsis. Among them, AtWIP1 and -2 are ubiquitously expressed in a partially overlapping pattern as revealed by both qRT-PCR and promoter:GUS assays. Subcellular localization analyses reveal that both AtWIP1 and -2 are localized at the plasma membrane while AtWIP1 shows a punctate distribution pattern. AtWIP1, -2 are transcriptionally induced by flg22 treatment, but repressed by effector(s) of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). Heterologous overexpression of GmWIP genes enhances resistance of Arabidopsis to Pst DC3000 at the cost of growth inhibition. Moreover, overexpression of GmWIP genes promotes pattern-triggered immunity (PTI) evidenced by increased expressions of flg22-inducible genes and enhanced seedling growth inhibition under flg22 treatment. Taken together, our results indicate that WIPs positively regulate plant resistance against Pst DC3000 by enhancing PTI responses.

• OSTI ID: 23103528
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 504, Issue 1; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ARABIDOPSIS
GLYCINE
POLYPEPTIDES
PSEUDOMONAS
SOYBEANS
TOMATOES