Assessment of Cellulose Nanofiber-Based Elastase Biosensors to Inflammatory Disease as a Function of Spacer Length and Fluorescence Response

Easson, Michael W.; Jordan, Jacobs H.; Edwards, J. Vincent; Prevost, Nicolette T.; Dupre, Rebecca A.; Hillyer, Matthew B.; Lima, Isabel M.; Nam, Sunghyun

doi:10.1021/acsabm.3c00885

Title: Assessment of Cellulose Nanofiber-Based Elastase Biosensors to Inflammatory Disease as a Function of Spacer Length and Fluorescence Response

Journal Article · Tue Feb 20 00:00:00 EST 2024 · ACS Applied Bio Materials

DOI:https://doi.org/10.1021/acsabm.3c00885· OSTI ID:2309791

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^[1]; Prevost, Nicolette T. ^[1];

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^[1]; Lima, Isabel M. ^[1];

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US Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, 1100 Allen Toussaint Blvd, New Orleans, Louisiana 70124, United States
Oak Ridge Institute for Science and Education, U.S. Department of Energy, 1299 Bethel Valley Rd, Oak Ridge, Tennessee 37831, United States

Inflammatory disease biomarker detection has become a high priority in point-of-care diagnostic research in relation to chronic wounds, with a variety of sensor-based designs becoming available. Herein, two primary aspects of biosensor design are examined: (1) assessment of a cellulose nanofiber (CNF) matrix derived from cotton ginning byproducts as a sensor transducer surface; and (2) assessment of the relation of spacer length and morphology between the CNF cellulose backbone and peptide fluorophore as a function of sensor activity for porcine pancreatic and human neutrophil elastases. X-ray crystallography, specific surface area, and pore size analyses confirmed the suitability of CNF as a matrix for wound care diagnostics. Based upon the normalized degree of substitution, a pegylated-linker connecting CNF transducer substrate to peptide fluorophore showed the greatest fluorescence response, compared to short- and long-chain alkylated linkers.

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Research Organization:: USDA Agricultural Research Service, New Orleans, LA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0014664

OSTI ID:: 2309791

Alternate ID(s):: OSTI ID: 2324897

Journal Information:: ACS Applied Bio Materials, Journal Name: ACS Applied Bio Materials Vol. 7 Journal Issue: 3; ISSN 2576-6422

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
elastase
biosensor
cellulose
nanofiber
proteases
inflammation
fluorescence
biotechnology
nitrogen
peptides and proteins
sensors

Title: Assessment of Cellulose Nanofiber-Based Elastase Biosensors to Inflammatory Disease as a Function of Spacer Length and Fluorescence Response

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