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Gut enterotype-dependent modulation of gut microbiota and their metabolism in response to xanthohumol supplementation in healthy adults

Journal Article · · Gut Microbes
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  1. Oregon State Univ., Corvallis, OR (United States)
  2. Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
  3. National University of Natural Medicine, Portland, OR (United States). Helfgott Research Institute; Univ. of California, San Diego, CA (United States)
  4. Oregon State Univ., Corvallis, OR (United States); National University of Natural Medicine, Portland, OR (United States). Helfgott Research Institute
+ Show Author Affiliations

Xanthohumol (XN), a polyphenol found in the hop plant (Humulus lupulus), has antioxidant, anti-inflammatory, prebiotic, and anti-hyperlipidemic activity. Preclinical evidence suggests the gut microbiome is essential in mediating these bioactivities; however, relatively little is known about XN’s impact on human gut microbiota in vivo. We conducted a randomized, triple-blinded, placebo-controlled clinical trial (ClinicalTrials.gov NCT03735420) to determine safety and tolerability of XN in healthy adults. Thirty healthy participants were randomized to 24 mg/day XN or placebo for 8 weeks. As secondary outcomes, quantification of bacterial metabolites and 16S rRNA gene sequencing were utilized to explore the relationships between XN supplementation, gut microbiota, and biomarkers of gut health. Although XN did not significantly change gut microbiota composition, it did re-shape individual taxa in an enterotype-dependent manner. High levels of inter-individual variation in metabolic profiles and bioavailability of XN metabolites were observed. Moreover, reductions in microbiota-derived bile acid metabolism were observed, which were enterotype-dependent. These results suggest interactions between XN and gut microbiota in healthy adults are highly inter-individualized and potentially indicate that XN elicits effects on gut health in an enterotype-dependent manner.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE; National Institutes of Health (NIH)
Grant/Contract Number:
AC05-76RL01830
OSTI ID:
2309679
Alternate ID(s):
OSTI ID: 2309680
Report Number(s):
PNNL-SA--194929
Journal Information:
Gut Microbes, Journal Name: Gut Microbes Journal Issue: 1 Vol. 16; ISSN 1949-0976
Publisher:
Taylor & FrancisCopyright Statement
Country of Publication:
United States
Language:
English

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59 BASIC BIOLOGICAL SCIENCES
Gut microbiota
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