Three-dimensional contractile muscle tissue consisting of human skeletal myocyte cell line

Journal Article · Sat Sep 15 04:00:00 EDT 2018 · Experimental Cell Research

DOI:https://doi.org/10.1016/J.YEXCR.2018.06.015· OSTI ID:23082660

Shima, Ai; Morimoto, Yuya ^[1]; Sweeney, H. Lee ^[2]; Takeuchi, Shoji ^[1]

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1. Institute of Industrial Science, The University of Tokyo, Tokyo (Japan)
2. Department of Pharmacology & Therapeutics, University of Florida College of Medicine, Gainesville, FL (United States)

Highlights: • Human skeletal myocyte cell line formed many myotubes in a fiber-based 3D tissue. • Myotubes in the 3D tissue started to contract spontaneously. • Myotubes in the 3D tissue contracted in response to exogenous electric stimuli. This paper describes a method to construct three-dimensional (3D) contractile human skeletal muscle tissues from a cell line. The 3D tissue was fabricated as a fiber-based structure and cultured for two weeks under tension by anchoring its both ends. While myotubes from the immortalized human skeletal myocytes used in this study never contracted in the conventional two-dimensional (2D) monolayer culture, myotubes in the 3D tissue showed spontaneous contraction at a high frequency and also reacted to the electrical stimulation. Immunofluorescence revealed that the myotubes in the 3D tissues had sarcomeres and expressed ryanodine receptor (RyR) and sarco/endoplasmic reticulum Ca{sup 2+}-ATPase (SERCA). In addition, intracellular calcium oscillations in the myotubes in the 3D tissue were observed. These results indicated that the 3D culture enabled the myocyte cell line to reach a more highly matured state compared to 2D culture. Since contraction is the most significant feature of skeletal muscle, we believe that our 3D human muscle tissue with the contractile ability would be a useful tool for both basic biology research and drug discovery as one of the muscle-on-chips.

• OSTI ID: 23082660
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 1 Vol. 370; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CALCIUM
CALCIUM IONS
ENDOPLASMIC RETICULUM
HUMAN POPULATIONS
RECEPTORS