Up-regulated miR-548k promotes esophageal squamous cell carcinoma progression via targeting long noncoding RNA-LET

Journal Article · Mon Jan 15 04:00:00 EST 2018 · Experimental Cell Research

DOI:https://doi.org/10.1016/J.YEXCR.2017.11.006· OSTI ID:23082532

Chen, Zhiyao; Lin, Jianqing; Wu, Shuhua; Xu, Chunhao; Chen, Feng; Huang, Zhijun ^[1]

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1. Department of Surgical Oncology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, 362000 (China)

Highlights: • miR-548k is significantly upregulated in ESCC and indicates poor prognosis. • miR-548k promotes the proliferation and migration of ESCC cells. • miR-548k directly targets and represses long noncoding RNA-LET (lncRNA-LET). • lncRNA-LET inhibits the proliferation and migration of ESCC cells. • The roles of miR-548k on ESCC are dependent on the negative regulation of lncRNA-LET. Dysregulated noncoding RNAs have been observed in diverse cancers. MIR458K is frequently amplified in esophageal squamous cell carcinoma (ESCC). However, the expression, clinical significances, and action mechanisms of miR-548k in ESCC are still unclear. In this study, we found that miR-548k is significantly up-regulated in ESCC tissues and cell lines. Up-regulated miR-548k expression is significantly correlated with advanced invasion depth, lymph node metastasis, advanced TNM stage, and poor overall survival. Gain-of- and loss-of-function assays demonstrated that miR-548k promotes the proliferation and migration of ESCC cells in vitro and tumor growth in vivo. Mechanistically, we found that miR-548k directly targets and represses the expression of long noncoding RNA-LET (lncRNA-LET), and further down-regulates p53 and up-regulates NF90. In addition, we found that lncRNA-LET is down-regulated and inversely correlated with miR-548k in ESCC. Down-regulated lncRNA-LET also indicated poor overall survival of ESCC patients. Functional assays demonstrated that lncRNA-LET inhibits the proliferation and migration of ESCC cells, and the effects of miR-548k on ESCC are dependent on the negative regulation of lncRNA-LET. In summary, our data revealed the critical roles of miR-548k-lncRNA-LET regulation axis in ESCC and suggested that the miR-548k-lncRNA-LET regulation axis may be promising prognostic biomarkers and therapeutic targets for ESCC.

• OSTI ID: 23082532
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 1 Vol. 362; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BIOLOGICAL MARKERS
CARCINOMAS
ESOPHAGUS
LYMPH NODES
METASTASES