MiR-155 targets PTCH1 to mediate endothelial progenitor cell dysfunction caused by high glucose
- Department of Peripheral Angiopathy, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, No. 26 of Heping Road, Harbin, Heilongjiang 150040 (China)
- Heilongjiang fire hospital, Harbin, Heilongjiang 150026 (China)
- Qiqihar Medical University, Qiqihar, Heilongjiang 161006 (China)
- Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040 (China)
Highlights: • MiR-155 was upregulated in EPCs of patients with DFU. • High glucose–induced upregulation of miR-155 in EPCs. • MiR-155 exacerbated EPCs dysfunction induced by high glucose. • MiR-155 targeted PTCH1 in EPCs. • MiR-155 impaired EPCs function via PTCH1 suppression. Endothelial progenitor cells (EPCs) are involved in diabetes-associated complications, including diabetic foot ulcer (DFU). Recent reports showed that miR-155 downregulation promotes wound healing in diabetic rats and ameliorates endothelial injury induced by high glucose, but its role in DFU is unknown. We found that miR-155 was overexpressed in EPCs from patients with DFU and in high glucose–induced EPCs from healthy people. Reductions in cell viability, migration, tube formation and nitric oxide production, as well as increases in lactated hydrogenase, cell apoptosis, and reactive oxygen species induced by high glucose, were enhanced by miR-155 overexpression and restrained by miR-155 inhibition. Additionally, dual-luciferase reporter assay demonstrated that miR-155 directly targeted the 3′ untranslated region of patched-1 (PTCH1), a receptor of the sonic hedgehog signaling pathway, and downregulated the mRNA and protein expression of PTCH1. qRT-PCR and Western blot results revealed that the PTCH1 was downregulated in EPCs treated with high glucose. Silencing PTCH1 by PTCH1 siRNA alleviated the protective effect of anti-miR-155 on high glucose–induced EPC dysfunction. Our results indicate that miR-155 worsened high glucose–induced EPC function by downregulating PTCH1. These findings suggest that miR-155 may be a potential therapeutic target for DFU.
- OSTI ID:
- 23082398
- Journal Information:
- Experimental Cell Research, Vol. 366, Issue 1; Other Information: Copyright (c) 2018 Published by Elsevier Inc.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
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