In situ mechanical characterization of a single cell-cell adhesion interface under large strain

Yang, Ruiguo; Lavrik, Nikolay V.; Esfahani, Amir Monemian; Rosenbohm, Jordan Daniel; Safa, Bahareh Tajvidi; Minnick, Grayson

In situ mechanical characterization of a single cell-cell adhesion interface under large strain

Patent · Tue Dec 19 04:00:00 EST 2023

OSTI ID:2293996

Yang, Ruiguo; Lavrik, Nikolay V.; Esfahani, Amir Monemian; Rosenbohm, Jordan Daniel; Safa, Bahareh Tajvidi; Minnick, Grayson

A method of measuring a stress-strain curve in a cell-cell adhesion interface, the method including: providing a structure including a first movable island supported by a first beam, a second movable island supported by a second beam, and a gap therebetween connected by a pair of cells forming a junction, the pair of cells comprising a cell-cell adhesion interface having an initial length defined by a distance between nuclei of the pair of cells; moving the second movable island with a defined displacement; determining a displacement of the first movable island based on moving the second movable island; calculating a difference between the displacement of the first movable island and the defined displacement of the second movable island based on moving the second movable island; determining an applied strain in the cell-cell adhesion interface between the pair of cells based on the difference divided by the initial length of the cell-cell adhesion interface; calculating a force between the cell-cell adhesion interface of the pair of cells based on the displacement of the first movable island; calculating a stress in the cell-cell adhesion interface between the pair of cells based on the force; and determining the stress-strain curve of the cell-cell adhesion interface between the pair of cells by plotting the calculated stress against the applied strain.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); NUtech Ventures, Lincoln, NE (United States)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH); National Science Foundation (NSF)

DOE Contract Number:: AC05-00OR22725

Assignee:: NUtech Ventures (Lincoln, NE); UT-Battelle, LLC (Oak Ridge, TN)

Patent Number(s):: 11,846,611

Application Number:: 17/473,090

OSTI ID:: 2293996

Country of Publication:: United States

Language:: English

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