Silver Nanoparticles Induced Cell Apoptosis, Membrane Damage of Azotobacter vinelandii and Nitrosomonas europaea via Generation of Reactive Oxygen Species

Journal Article · Mon Jul 15 04:00:00 EDT 2019 · Bulletin of Environmental Contamination and Toxicology

DOI:https://doi.org/10.1007/S00128-019-02622-0· OSTI ID:22933505

Zhang, Li; Wu, Lingli; Mi, Yazhu; Si, Youbin ^[1]

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1. Anhui Agricultural University, Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment (China)

Silver nanoparticles (AgNPs) is widely used as an antibacterial agent, but the specific antibacterial mechanism is still conflicting. This study aimed to investigate the size dependent inhibition of AgNPs and the relationship between inhibition and reactive oxygen species (ROS). Azotobactervinelandii and Nitrosomonaseuropaea were exposed to AgNPs with different particles size (10 nm and 50 nm). The ROS production was measured and the results showed that the generation of ROS related to the particle size and concentrations of AgNPs. At 10 mg/L of 10 nm Ag particles, the apoptosis rate of A. vinelandii and N. europaea were 20.23% and 1.87% respectively. Additionally, the necrosis rate of A. vinelandii and N. europaea reached to 15.20% and 42.20% respectively. Furthermore, transmission electron microscopy images also indicated that AgNPs caused severely bacterial cell membrane damage. Together these data suggested that the toxicity of AgNPs depends on its particle size and overproduction of ROS.

• OSTI ID: 22933505
• Journal Information: Bulletin of Environmental Contamination and Toxicology, Journal Name: Bulletin of Environmental Contamination and Toxicology Journal Issue: 1 Vol. 103; ISSN 0007-4861; ISSN BECTA6
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
AZOTOBACTER
CELL MEMBRANES
ECOLOGICAL CONCENTRATION
NANOPARTICLES
NECROSIS
OXYGEN
PARTICLE SIZE
SILVER
TOXICITY
TRANSMISSION ELECTRON MICROSCOPY