Resistance and reduction potential of serratia marcescens in hexavalent chromium - 15721
- Department of Biology, Claflin University, Orangeburg, SC (United States)
Heavy metal pollution due to legacy waste from the Cold War nuclear proliferation remains a huge problem at federal and industrial sites. Microbial bioremediation is a cost effective method for removing heavy metals from soil and groundwater. This pilot research project looks at the reduction of chromium present at DOE's Savannah River Site and the ability of bacteria population to aid in the reduction of heavy metals. The purpose of this study was to characterize the growth pattern of Serratia marcescens strain 93-1399-1 in the presence of heavy metal hexavalent chromium (Cr [VI]) and its ability to convert Cr (VI) to the less toxic Cr (III). When S. marcescens was grown on LB agar with increasing concentrations of Cr (VI) as potassium dichromate, the bacteria survived at all concentrations tested up to 200 ppm. Growth curve of S. marcescens in Cr (VI) showed that the bacteria exposed to 25 pm grew in similarly to bacteria without Cr (VI), had reduced exponential growth at 50 and 100 ppm and the poor growth in 200 pp after 24 hours. Plate count assays showed that the 65% of the bacteria survived in 25 ppm of Cr (VI) but only 5% survived at 50 ppm. In preliminary chromium reduction assays, S. marcescens was grown for 24 hours in 25 ppm of Cr (VI). S. marcescens reduced 60% of the Cr (VI) to Cr (III) as compared to 4% reduction in broth alone. These data suggest that the S. marcescens strain may be useful in bio-remediating Cr (VI) at contaminated sites. Future studies will focus on identifying the genes responsible for Cr (VI) reduction. (authors)
- Research Organization:
- WM Symposia, Inc., PO Box 27646, 85285-7646 Tempe, AZ (United States)
- OSTI ID:
- 22824533
- Report Number(s):
- INIS-US--19-WM-15721
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
54 ENVIRONMENTAL SCIENCES
AGAR
BIOREMEDIATION
CHROMIUM
CONCENTRATION RATIO
COST EFFECTIVENESS ANALYSIS
DICHROMATES
GROUND WATER
HEAVY METALS
POLLUTION
POTASSIUM COMPOUNDS
PROLIFERATION
SAVANNAH RIVER
SERRATIA
SOILS
TOXICITY