Statistical 3D morphology characterization of vaterite microspheres produced by engineered Escherichia coli

Lin, Alex Y.W.; Wu, Zong-Yen; Pattison, Alexander J.; Müller, Isaak E.; Yoshikuni, Yasuo; Theis, Wolfgang; Ercius, Peter

doi:10.1016/j.bioadv.2023.213711

Title: Statistical 3D morphology characterization of vaterite microspheres produced by engineered Escherichia coli

Journal Article · Mon Nov 27 00:00:00 EST 2023 · Biomaterials Advances (Online)

DOI:https://doi.org/10.1016/j.bioadv.2023.213711· OSTI ID:2281554

Lin, Alex Y.W. ^[1]; Wu, Zong-Yen ^[2]; Pattison, Alexander J. ^[1]; Müller, Isaak E. ^[2]; Yoshikuni, Yasuo ^[2]; Theis, Wolfgang ^[3]; Ercius, Peter ^[1]

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Molecular Foundry
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). USDOE Joint Genome Institute (JGI)
University of Birmingham (United Kingdom)

Hollow vaterite microspheres are important materials for biomedical applications such as drug delivery and regenerative medicine owing to their biocompatibility, high specific surface area, and ability to encapsulate a large number of bioactive molecules and compounds. We demonstrated that hollow vaterite microspheres are produced by an Escherichia coli strain engineered with a urease gene cluster from the ureolytic bacteria Sporosarcina pasteurii in the presence of bovine serum albumin. We characterized the 3D nanoscale morphology of five biogenic hollow vaterite microspheres using 3D high-angle annular dark field scanning transmission electron microscopy (HAADF-STEM) tomography. Using automated high-throughput HAADF-STEM imaging across several sample tilt orientations, we show that the microspheres evolved from a smaller more ellipsoidal shape to a larger more spherical shape while the internal hollow core increased in size and remained relatively spherical, indicating that the microspheres produced by this engineered strain likely do not contain the bacteria. The statistical 3D morphology information demonstrates the potential for using biogenic calcium carbonate mineralization to produce hollow vaterite microspheres with controlled morphologies.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); USDOE Laboratory Directed Research and Development (LDRD) Program

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2281554

Journal Information:: Biomaterials Advances (Online), Vol. 156; ISSN 2772-9508

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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