DNA transfer between two different species mediated by heterologous cell fusion in Clostridium coculture

Charubin, Kamil; Hill, John D.; Papoutsakis, Eleftherios Terry; Lovley, ed., Derek R.

doi:10.1128/mbio.03133-23

DNA transfer between two different species mediated by heterologous cell fusion in Clostridium coculture

Journal Article · Wed Feb 14 00:00:00 EST 2024 · mBio (Online)

DOI:https://doi.org/10.1128/mbio.03133-23· OSTI ID:2281037

Charubin, Kamil ^[1]; ^[1]; ^[1]; Lovley, ed., Derek R.

Department of Chemical and Biomolecular Engineering, The Delaware Biotechnology Institute, University of Delaware, Newark, Delaware, USA

Investigations of natural multispecies microbiomes and synthetic microbial cocultures are attracting renewed interest for their potential application in biotechnology, ecology, and medical fields. Previously, we have shown the syntrophic coculture of C. acetobutylicum and C. ljungdahlii undergoes heterologous cell-to-cell fusion, which facilitates the exchange of cytoplasmic protein and RNA between the two organisms. We now show that heterologous cell fusion between the two Clostridium organisms can facilitate the exchange of DNA. By applying selective pressures to this coculture system, we isolated clones of wild-type C. acetobutylicum which acquired the erythromycin resistance (erm) gene from the C. ljungdahlii strain carrying a plasmid with the erm gene. Single-molecule real-time sequencing revealed that the erm gene was integrated into the genome in a mosaic fashion. Our data also support the persistence of hybrid C. acetobutylicum / C. ljungdahlii cells displaying hybrid DNA-methylation patterns.

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Research Organization:: Univ. of Delaware, Newark, DE (United States)

Sponsoring Organization:: National Institute of General Medical Sciences (NIGMS); National Institutes of Health (NIH); National Science Foundation (NSF); US Army Research Office (ARO); USDOE Advanced Research Projects Agency - Energy (ARPA-E)

Grant/Contract Number:: AR0001505

OSTI ID:: 2281037

Alternate ID(s):: OSTI ID: 2472240

Journal Information:: mBio (Online), Journal Name: mBio (Online) Journal Issue: 2 Vol. 15; ISSN 2150-7511

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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DNA transfer between two different species mediated by heterologous cell fusion in Clostridium coculture

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