Charge Transfer Dissociation (CTD) Mass Spectrometry of Peptide Cations: Study of Charge State Effects and Side-Chain Losses

Journal Article · Sat Jul 15 04:00:00 EDT 2017 · Journal of the American Society for Mass Spectrometry

DOI:https://doi.org/10.1007/S13361-016-1574-Y· OSTI ID:22777065

Li, Pengfei

1+, 2+, and 3+ precursors of substance P and bradykinin were subjected to helium cation irradiation in a 3D ion trap mass spectrometer. Charge exchange with the helium cations produces a variety of fragment ions, the number and type of which are dependent on the charge state of the precursor ions. For 1+ peptide precursors, fragmentation is generally restricted to C−CO backbone bonds (a and x ions), whereas for 2+ and 3+ peptide precursors, all three backbone bonds (C−CO, C−N, and N−Cα) are cleaved. The type of backbone bond cleavage is indicative of possible dissociation channels involved in CTD process, including high-energy, kinetic-based, and ETD-like pathways. In addition to backbone cleavages, amino acid side-chain cleavages are observed in CTD, which are consistent with other high-energy and radical-mediated techniques. The unique dissociation pattern and supplementary information available from side-chain cleavages make CTD a potentially useful activation method for the structural study of gas-phase biomolecules. .

• OSTI ID: 22777065
• Journal Information: Journal of the American Society for Mass Spectrometry, Journal Name: Journal of the American Society for Mass Spectrometry Journal Issue: 7 Vol. 28; ISSN 1044-0305; ISSN JAMSEF
• Country of Publication: United States
• Language: English

Related Subjects

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
74 ATOMIC AND MOLECULAR PHYSICS
CARBON MONOXIDE
CHARGE EXCHANGE
CHARGE STATES
DISSOCIATION
ELECTRON TRANSFER
FRAGMENTATION
HELIUM
IRRADIATION
MASS SPECTROMETERS
MASS SPECTROSCOPY
RADICALS
TRAPS