The Area Between Exchange Curves as a Measure of Conformational Differences in Hydrogen-Deuterium Exchange Mass Spectrometry Studies

Journal Article · Mon May 15 00:00:00 EDT 2017 · Journal of the American Society for Mass Spectrometry

DOI:https://doi.org/10.1007/S13361-017-1615-1· OSTI ID:22777002

Weber, Daniel P. ^[1]

---

1. Interclypse (United States)

Hydrogen-deuterium exchange mass spectrometry (HDX-MS) provides information about protein conformational mobility under native conditions. The area between exchange curves, A{sub bec}, a functional data analysis concept, was adapted to the interpretation of HDX-MS data and provides a useful measure of exchange curve dissimilarity for tests of significance. Importantly, for most globular proteins under native conditions, A{sub bec} values provide an estimate of the log ratio of exchange-competent fractions in the two states, and thus are related to differences in the free energy of microdomain unfolding. .

• OSTI ID: 22777002
• Journal Information: Journal of the American Society for Mass Spectrometry, Vol. 28, Issue 5; Conference: 28. ASMS Sanibel conference on mass spectrometry: Characterization of protein therapeutics by mass spectrometry, Clearwater Beach, FL (United States), 21-24 Jan 2016; Other Information: Copyright (c) 2017 American Society for Mass Spectrometry; Article Copyright (c) 2017 American Society for Mass Spectrometry (outside the USA); http://www.springer-ny.com; Country of input: International Atomic Energy Agency (IAEA); ISSN 1044-0305
• Country of Publication: United States
• Language: English

Related Subjects

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
DATA ANALYSIS
DEUTERIUM
DIAGRAMS
FREE ENERGY
HYDROGEN
ION MOBILITY
MASS SPECTROSCOPY
PROTEINS