Biocrystallization in Bacterial and Fungal Cells and Spores
Journal Article
·
· Crystallography Reports
- Russian Academy of Sciences, Semenov Institute of Chemical Physics (Russian Federation)
- Russian Academy of Sciences, Federal Research Centre “Fundamentals of Biotechnology,” (Russian Federation)
- Moscow State University (Russian Federation)
- European Synchrotron Radiation Facility (France)
A series of X-ray diffraction experiments were performed for the first time to study stress-induced biocrystallization (structural response to stress) in the bacteria E. coli, the spore-forming bacteria Bacillus cereus, and in cells and spores of the mycelial fungus Umbelopsis ramanniana. High-intensity areas with spacings of 90 and 44 Å are indicative of a periodically ordered arrangement (most likely nanocrystalline) of the bacterial nucleoid. For the starved bacteria Bacillus cereus, a peak at a spacing of 45 Å is also assigned to nanocrystalline complexes of DNA with the Dps protein. The spores of the fungus Umbelopsis ramanniana VKM F-582, as well as the spores of Bacillus cereus, form ordered arrays of DNA molecules with DNA-condensing acid-soluble proteins SASPs. Starved dehydrated mycelial cells of the fungus Umbelopsis ramanniana form ordered structures with spacings from 27 to 55 Å. A series of peaks reflect the formation of a number of ordered protein arrays, apparently with DNA, with continuously varying characteristic interplanar spacings.
- OSTI ID:
- 22758218
- Journal Information:
- Crystallography Reports, Journal Name: Crystallography Reports Journal Issue: 4 Vol. 63; ISSN 1063-7745; ISSN CYSTE3
- Country of Publication:
- United States
- Language:
- English
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