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Interrelationships among methods of estimating microbial biomass across multiple soil orders and biomes: Supporting data

Dataset ·
 [1];  [2];  [3];  [4]; ; ;  [5];  [6];  [4];  [4]
  1. University of Minnesota, Saint Paul, MN (United States); Oak Ridge National Laboratory
  2. Tetra Tech, Inc., Pasadena, CA (United States)
  3. Roanoke College
  4. Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
  5. Argonne National Laboratory (ANL), Argonne, IL (United States)
  6. University of Minnesota, Saint Paul, MN (United States)
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This dataset contains environmental and soil measurements from 18 different locations across the globe including the SPRUCE experiment site and multiple sampling depths, with 17 of these locations having samples processed between 2012-2013 and one location (SPRUCE) collected in 2021 and processed in 2022. Environmental measurements include: mean annual temperature, mean annual precipitation, and 30-day presampling temperature. Soil physicochemical measurements include: particle size analysis (PSA), pH, gravimetric moisture content (GMC), bulk soil carbon (C) and nitrogen (N), total organic C and N, C:N ratio, and dissolved organic carbon (DOC). Soil biological measurements include: microbial biomass carbon (MBC) measured through chloroform fumigation extraction (CFE), gene copy numbers (GCN) of bacteria, fungi, and archaea measured through quantitative polymerase chain reaction (qPCR), DNA yield measured through Nanodrop spectrophotometry, and phospholipid fatty acids (PLFA) of bacteria and fungi measured through PLFA analysis. This data set contains one file in comma separate (*.csv) format.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science (BSS)
DOE Contract Number:
AC05-00OR22725
OSTI ID:
2274949
Country of Publication:
United States
Language:
English

References (3)

Global, Regional, and National Fossil-Fuel CO2 Emissions (1751 - 2014) (V. 2017) dataset January 2017
Organic-matter decomposition along a temperature gradient in a forested headwater stream journal June 2016
Influence of dual nitrogen and phosphorus additions on nutrient uptake and saturation kinetics in a forested headwater stream journal December 2018

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Related Subjects

C:N ratio
Nanodrop spectrophotometry
PLFA
Particle size analysis
bulk soil carbon
bulk soil nitrogen
chloroform fumigation extraction
dissolved organic carbon (DOC)
gene copy numbers
gravimetric moisture content
microbial biomass carbon
pH
qPCR
total organic C
total organic N