Curcumin inhibits metastasis in human papillary thyroid carcinoma BCPAP cells via down-regulation of the TGF-β/Smad2/3 signaling pathway

Journal Article · Mon Feb 15 04:00:00 EST 2016 · Experimental Cell Research

DOI:https://doi.org/10.1016/J.YEXCR.2016.01.006· OSTI ID:22746413

Zhang, Li ^[1]; Cheng, Xian; Gao, Yanyan ^[2]; Zhang, Chiyu ^[3]; Bao, Jiandong ^[2]; Guan, Haixia ^[4]; Yu, Huixin ^[2]; Lu, Rongrong ^[3]; Xu, Qiang ^[1]; Sun, Yang ^[1]

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1. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu (China)
2. Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu (China)
3. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu (China)
4. Department of Endocrinology & Metabolism and Institute of Endocrinology, The First Hospital of China Medical University, Shenyang, Liaoning (China)

Thyroid cancers usually possess a good prognosis while the risks of recurrence and metastasis turn out to be a disturbing issue. Curcumin [bis(4-hydroxy-3-methoxy-phenyl)-1,6-heptadiene-3,5-dione] is a natural polyphenolic compound mainly found in turmeric (Curcuma longa). Our previous studies have demonstrated that curcumin showed proliferation-inhibitory and apoptosis-inducing effects on K1 papillary thyroid cancer cells. However, the mechanism underlying the inhibition effects of curcumin on thyroid cancer cells remains unclear. Herein, we demonstrated that curcumin remarkably increased the expression of the epithelial marker E-cadherin and repressed the expression of the mesenchymal marker vimentin in human papillary thyroid carcinoma BCPAP cells. Curcumin also suppressed multiple metastatic steps of BCPAP cells, including cell attachment, spreading as well as migration. In addition, the transcription, secretion and activation of matrix metalloproteinases (MMPs) induced by transforming growth factor-β1 (TGF-β1) in BCPAP cells were mitigated upon curcumin treatment. Further evidence showed that curcumin decreased TGF-β1-mediated phosphorylation of Smad2 and Smad3. These results revealed that curcumin inhibited the TGF-β1-induced epithelial–mesenchymal transition (EMT) via down-regulation of Smad2/3 signaling pathways. Our findings provide new evidence that the anti-metastatic and anti-EMT activities of curcumin may contribute to the development of chemo-preventive agents for thyroid cancer treatment. - Highlights: • Curcumin inhibits the adhesion, spreading and migration of BCPAP cells. • Curcumin inhibits TGF-β1-induced EMT in BCPAP cells. • Curcumin inhibits TGF-β/Smad2/3 signaling pathway in BCPAP cells.

• OSTI ID: 22746413
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 2 Vol. 341; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADHESION
ANIMAL TISSUES
APOPTOSIS
CARCINOMAS
CELL PROLIFERATION
CONCANAVALIN A
CURCUMIN
DMSO
GROWTH FACTORS
HAZARDS
HUMAN POPULATIONS
INHIBITION
LYMPH
LYMPH NODES
MATRICES
METASTASES
MIGRATION
PHOSPHORYLATION
PLANT GROWTH
SECRETION
SIGNALS
THYROID
TRANSCRIPTION