Two single mutations in the fusion protein of Newcastle disease virus confer hemagglutinin-neuraminidase independent fusion promotion and attenuate the pathogenicity in chickens

Journal Article · Fri Sep 15 04:00:00 EDT 2017 · Virology

DOI:https://doi.org/10.1016/J.VIROL.2017.06.021· OSTI ID:22722965

Ji, Yanhong; Liu, Tao; Jia, Yane; Liu, Bin ^[1]; Yu, Qingzhong ^[2]; Cui, Xiaole; Guo, Fengfeng ^[1]; Chang, Huiyun ^[1]; Zhu, Qiyun ^[1]

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1. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046 (China)
2. United States Department of Agriculture, Agriculture Research Service, US National Poultry Research Center, Southeast Poultry Research Laboratory, Athens, GA 30605 (United States)

The fusion (F) protein of Newcastle disease virus (NDV) affects viral infection and pathogenicity through mediating membrane fusion. Previously, we found NDV with increased fusogenic activity in which contained T458D or G459D mutation in the F protein. Here, we investigated the effects of these two mutations on viral infection, fusogenicity and pathogenicity. Syncytium formation assays indicated that T458D or G459D increased the F protein cleavage activity and enhanced cell fusion with or without the presence of HN protein. The T458D- or G459D-mutated NDV resulted in a decrease in virus replication or release from cells. The animal study showed that the pathogenicity of the mutated NDVs was attenuated in chickens. These results indicate that these two single mutations in F altered or diminished the requirement of HN for promoting membrane fusion. The increased fusogenic activity may disrupt the cellular machinery and consequently decrease the virus replication and pathogenicity in chickens. - Highlights: • T458D or G459D mutation in the F protein enhanced the fuosogenicity with HN. • T458D or G459D mutation in the F protein promoted the fusion independently. • The growth of the T458D or G459D mutated virus was decreased in the cells. • The pathogenicity of the T458D or G459D mutated virus was attenuated in chickens.

• OSTI ID: 22722965
• Journal Information: Virology, Journal Name: Virology Vol. 509; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CHICKENS
MUTANTS
MUTATIONS
NEWCASTLE DISEASE
PLANT GROWTH
VIRUSES