Maternal trans-general analysis of the human mitochondrial DNA pattern

Journal Article · Sat Nov 04 04:00:00 EDT 2017 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2017.08.138· OSTI ID:22719132

Han, Xiao; Zhao, Zitong; Zhang, Minjie; Li, Guochao ^[1]; Yang, Caiyun; Du, Fengxia; Wang, Junyun ^[1]; Zhang, Yan ^[2]; Wang, Yuanyuan ^[3]; Jia, Yongsheng ^[4]; Li, Binghui ^[3]; Sun, Yingli ^[1]

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1. Key Laboratory of Genomic and Precision Medicine, China Gastrointestinal Cancer Research Center, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101 (China)
2. College of Bioinformatics Science and Technology, Harbin Medical University, Harbin 150081 (China)
3. Laboratory of Cancer Cell Biology, Key Laboratory of Breast Cancer Prevention and Therapy, National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060 (China)
4. Department of Breast Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Breast Cancer Prevention and Therapy, Ministry of Education, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University, Tianjin 300060 (China)

There is an intimate connection between mitochondrial DNA (mtDNA) methylation and some diseases, such as cancer. MtDNA is almost strictly maternally inherited. However, whether the aberrant mtDNA methylation involved in breast cancer progression and whether mtDNA methylation can be transmitted through maternal line are poorly understood. Here we applied bisulfite sequencing to global mitochondrial DNA and whole genomic DNA methylation array from fifteen members of five three-female-generation families with one breast cancer patient in each family. We found that mtDNA methylation was maternally inherited in D-loop region and eight aberrant mtDNA methylation sites were correlated with breast cancer. Furthermore, conjoint analysis showed that mtDNA methylation sites could be potential biomarkers combined with nuclear DNA methylation sites for breast cancer risk prediction. - Highlights: • MtDNA methylation was modestly maternally inherited, especially in D-loop region. • Eight aberrant mtDNA methylation sites were correlated with breast cancer. • Nine nDNA methylation sites were identified as potential breast cancer-related sites. • MtDNA-nDNA methylation sites could be potential biomarkers for breast cancer. • A hypothetical model of mtDNA methylation maternal inheritance was proposed.

• OSTI ID: 22719132
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 493; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BIOLOGICAL MARKERS
DNA
HAZARDS
MAMMARY GLANDS
METHYLATION
MITOCHONDRIA
NEOPLASMS
PATIENTS