Suppression of CD36 attenuates adipogenesis with a reduction of P2X7 expression in 3T3-L1 cells

Journal Article · Sat Sep 09 04:00:00 EDT 2017 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2017.07.077· OSTI ID:22719072

Gao, Huanqing ^[1]; Li, Danyang; Yang, Ping; Zhao, Lei; Wei, Li ^[1]; Chen, Yaxi ^[1]

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1. Centre for Lipid Research & Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, 400016 Chongqing (China)

Adipogenesis is a process of differentiation from preadipocyte into adipocyte, and is regulated by several transcription factors, including the peroxisome proliferator-activated receptor gamma (PPARγ) and the CCAAT-enhancer-binding protein alpha (C/EBPα). CD36 is a membrane protein which contributes to the metabolic disorders such as obesity. Although the previous study demonstrated CD36 participated in the progression of adipogenesis, the mechanism is still unclear. We report here that knockdown of CD36 expression by CD36 small interfering RNA (siRNA) resulted in a reduction of adipocyte differentiation and adipogenic protein expression. In addition, purinergic receptor P2X, ligand-gated ion channel 7 (P2X7) was downregulated in CD36-knockdown 3T3-L1 cells, suggesting that the suppression of CD36 attenuates adipogenesis via the P2X7 pathway in 3T3-L1 cells. - Highlights: • CD36 regulated adipogenesis in 3T3-L1 cells. • Suppression of CD36 downregulated the expression of adipogenic gene. • P2X7 was downregulated in CD36-knockdown 3T3-L1 cells.

• OSTI ID: 22719072
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 491; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ATP
GENES
INHIBITION
LIGANDS
METABOLIC DISEASES
RECEPTORS
REDUCTION
RNA
TRANSCRIPTION
TRANSCRIPTION FACTORS