miR-141-3p inhibits fibroblast proliferation and migration by targeting GAB1 in keloids

Journal Article · Sat Aug 19 04:00:00 EDT 2017 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2017.06.040· OSTI ID:22719033

Feng, Jingjuan ^[1]; Xue, Siliang ^[2]; Pang, Qiuyu; Rang, Zhen ^[3]; Cui, Fan ^[3]

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1. Department of Anorectal Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072 (China)
2. Department of Dermatology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041 (China)
3. Institute of Dermatology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072 (China)

Keloids are benign dermal fibroproliferative tumors that develop as a result of several dysregulated processes. Emerging evidence has revealed that miRNAs contribute to keloid formation. However, the molecular mechanisms of keloid pathogenesis remain unclear. In our study, we found that miR-141-3p in keloid tissues and keloid fibroblasts was significantly decreased compared with the levels in normal tissues and normal skin fibroblasts, respectively. miR-141-3p overexpression resulted in significantly decreased proliferation and migration and the promotion of apoptosis in keloid fibroblasts, whereas miR-141-3p knockdown in keloid fibroblasts yielded the opposite results. Growth factor receptor binding 2-associated binding protein 1 (GAB1) was identified and confirmed as a direct target of miR-141-3p. The expression of GAB1 was up-regulated in keloid tissues, and the restoration of GAB1 partially reversed the inhibitory effects of miR-141-3p on the proliferation and migration of keloid fibroblasts. All data suggested that miR-141-3p decreased the proliferation and migration of keloid fibroblasts by repressing GAB1 expression, providing a useful target for keloid management. - Highlights: • miR-141-3p is down-regulated in human keloids and suppresses keloid fibroblast proliferation. • miR-141-3p induces keloid fibroblast apoptosis and inhibits cell migration. • GAB1 is a direct target of miR-141-3p. • miR-141-3p inhibits keloid fibroblast proliferation and migration by negatively regulating GAB1 expression.

• OSTI ID: 22719033
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 490; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANIMAL TISSUES
APOPTOSIS
BIOLOGICAL RECOVERY
FIBROBLASTS
GROWTH FACTORS
NEOPLASMS
PATHOGENESIS
RECEPTORS
SKIN