Graphene modified titanium alloy promote the adhesion, proliferation and osteogenic differentiation of bone marrow stromal cells

Journal Article · Sat Jul 22 04:00:00 EDT 2017 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2017.05.124· OSTI ID:22719019

Li, Kewen ^[1]; Yan, Jinhong ^[1]; Wang, Chunhui ^[2]; Bi, Long; Zhang, Qi ^[1]; Han, Yisheng ^[1]

---

1. Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an (China)
2. Department of Military Medical Training, Urumqi Ethnic Cadre College, Hutubi, Xinjiang 831200 (China)

We studied the effects of graphene coating on improving the biological activity of a titanium alloy (Ti{sub 6}Al{sub 4}V) widely used in hip and knee joint replacements. The experiments included immunofluorescence staining for observing cellular adhesion, Cell Counting Kit-8 (CCK-8) for evaluating cellular proliferation and reverse transcription-polymerase chain reaction (RT-PCR) for detecting the differentiation of bone marrow stromal cells on different scaffolds. The results showed that G-Ti{sub 6}Al{sub 4}V exhibited a higher mean integrated optical density (IOD) for vinculin and resulted in a higher cell proliferation rate and higher osteoblast-specific gene transcription levels. In summary, graphene could be used as a new nanocoating material for Ti{sub 6}Al{sub 4}V scaffolds to enhance their surface bioactivity. - Highlights: • Graphene coating could promote the adhesion and proliferation of SD rats' BMSCs. • Graphene coating could accelerate BMSCs specific differentiation into osteogenic lineages. • Graphene coating could enhance the surface bioactivity of Ti-based alloys.

• OSTI ID: 22719019
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 489; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BONE JOINTS
BONE MARROW
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
GRAPHENE
POLYMERASE CHAIN REACTION
POLYMERASES
RATS
TITANIUM
TITANIUM ALLOYS
TRANSCRIPTION