The miR-599 promotes non-small cell lung cancer cell invasion via SATB2

Journal Article · Sat Mar 25 04:00:00 EDT 2017 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2017.02.005· OSTI ID:22696922

Tian, Wenjun ^[1]; Wang, Guanghai ^[2]; Liu, Yiqing ^[1]; Huang, Zhenglan ^[3]; Zhang, Caiqing; Ning, Kang; Yu, Cuixiang ^[2]; Shen, Yajuan; Wang, Minghui; Li, Yuantang; Wang, Yong; Zhang, Bingchang ^[1]; Zhao, Yaoran ^[1]

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1. Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan (China)
2. Department of Respiratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan (China)
3. Key Laboratory of Medical Diagnostics of Ministry of Education, Faculty of Laboratorial Medicine, Chongqing Medical University, Chongqing (China)

MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. Here, we identified that miR-599 is up-regulated in non-small cell lung cancer (NSCLC) patients. It promoted NSCLC cell proliferation by negatively regulating SATB2. In NSCLC cell lines, CCK-8 proliferation assay indicated that the cell proliferation is promoted by miR-599 mimics. Transwell assay showed that miR-599 mimics promoted the invasion and migration of NSCLC cells. Luciferase assays confirmed that miR-599 directly binds to the 3'untranslated region of SATB2, and western blotting showed that miR-599 suppresses the expression of SATB2 at the protein level. This study indicates that miR-599 promotes proliferation and invasion of NSCLC cell lines via SATB2. The miR-599 may represent a potential therapeutic target for NSCLC treatment. - Highlights: • miR-599 is up-regulated in NSCLC. • miR-599 promotes the proliferation and invasion of NSCLC cells. • miR-599 inhibitors inhibits the proliferation and invasion of NSCLC cells. • miR-599 targets 3′ UTR of SATB2 in NSCLC cells. • miR-599 inhibits SATB2 in NSCLC cells.

• OSTI ID: 22696922
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 485; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL PROLIFERATION
INHIBITION
LUNGS
NEOPLASMS