Silencing myotubularin related protein 7 enhances proliferation and early differentiation of C2C12 myoblast

Journal Article · Sat Mar 11 04:00:00 EST 2017 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2017.01.143· OSTI ID:22696903

Yuan, Zhuning; Chen, Yaosheng; Zhang, Xumeng; Zhou, Xingyu; Li, Mingsen; Chen, Hu; Wu, Ming; Zhang, Ying; Mo, Delin

Myotubularin related protein 7 (MTMR7) is a key member of the highly conserved myotubularin related proteins (MTMRs) family, which has phosphatase activity. MTMR7 was increased during myoblast differentiation and exhibited high expression level at primary fibers formation stages in pigs. This suggests that MTMR7 may be involved in myogenesis. In our study, we investigated the roles of MTMR7 on proliferation and differentiation of C2C12 myoblasts. Knocking down MTMR7 not only enhanced myoblast early differentiation via altering the expression of Myf5, but also promoted myoblast proliferation through increasing cyclinA2 expression. The improved proliferation capacity was related to the increased phosphorylation of AKT. Taken together, our research demonstrates that MTMR7 plays an important role in proliferation and early differentiation of C2C12 myoblast. - Highlights: • The expression pattern of MTMR7 suggests that MTMR7 may be involved in myogenesis. • Silencing MTMR7, by siRNA, enhances myoblast early differentiation via increasing the expression of Myf5. • Silencing MTMR7 promotes myoblast proliferation likely through AKT signaling pathway.

• OSTI ID: 22696903
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 484; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AUGMENTATION
CAPACITY
DEOXYURIDINE
FIBERS
IODIDES
MYOBLASTS
MYOSIN
PHOSPHATASES
PHOSPHORYLATION
PLANT GROWTH
POLYMERASE CHAIN REACTION
PROLIFERATION
SIGNALS
SWINE