skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: microRNA-495 promotes bladder cancer cell growth and invasion by targeting phosphatase and tensin homolog

Abstract

Accumulating evidence has linked deregulation of microRNA-495 (miR-495) to tumorigenesis; however, its function in tumor progression is controversial. This work was undertaken to explore the expression and biological roles of miR-495 in bladder cancer. The expression of miR-495 was examined in 67 pairs of bladder cancer and adjacent normal bladder tissues. The roles of miR-495 in bladder cancer cell proliferation and invasion in vitro and tumorigenesis in vivo were determined. Direct target gene(s) mediating the activity of miR-495 in bladder cancer cells was identified. It was found that miR-495 was expressed at greater levels in bladder tissues and cell lines. High expression of miR-495 was significantly associated with larger tumor size, advanced TNM stage, and lymph node metastasis. Overexpression of miR-495 significantly promoted bladder cancer cell proliferation and invasion, whereas inhibition of miR-495 suppressed cell proliferation and invasion. PTEN, a well-defined tumor suppressor was identified to be a target gene of miR-495. A significant inverse correlation between miR-495 and PTEN expression was noted in bladder cancer tissues (r = −0.3094, P = 0.0125). Overexpression of miR-495 led to reduction of PTEN expression in bladder cancer cells. Rescue experiments showed that enforced expression of PTEN impaired miR-495-mediated bladder cancer proliferation and invasion. In vivo mouse studies demonstrated thatmore » overexpression of miR-495 accelerated the growth of subcutaneous bladder cancer xenografts, which was associated with downregulation of PTEN. Overall, these findings indicate that miR-495 upregulation contributes to bladder cancer cell growth, invasion, and tumorigenesis by targeting PTEN and offer a potential therapeutic target for bladder cancer. - Highlights: • miR-495 upregulation induces aggressive phenotype in bladder cancer. • miR-495 is inversely correlated with PTEN in bladder cancer. • miR-495 promotes bladder cancer cell proliferation and invasion by targeting PTEN.« less

Authors:
; ; ; ; ; ;
Publication Date:
OSTI Identifier:
22696855
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 483; Journal Issue: 2; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; BLADDER; CELL PROLIFERATION; IN VIVO; INHIBITION; LYMPH NODES; NEOPLASMS; PLANT GROWTH; PLANT TISSUES

Citation Formats

Tan, Mingyue, Mu, Xingyu, Liu, Zhihong, Tao, Le, Wang, Jun, Ge, Jifu, and Qiu, Jianxin. microRNA-495 promotes bladder cancer cell growth and invasion by targeting phosphatase and tensin homolog. United States: N. p., 2017. Web. doi:10.1016/J.BBRC.2017.01.019.
Tan, Mingyue, Mu, Xingyu, Liu, Zhihong, Tao, Le, Wang, Jun, Ge, Jifu, & Qiu, Jianxin. microRNA-495 promotes bladder cancer cell growth and invasion by targeting phosphatase and tensin homolog. United States. doi:10.1016/J.BBRC.2017.01.019.
Tan, Mingyue, Mu, Xingyu, Liu, Zhihong, Tao, Le, Wang, Jun, Ge, Jifu, and Qiu, Jianxin. Sun . "microRNA-495 promotes bladder cancer cell growth and invasion by targeting phosphatase and tensin homolog". United States. doi:10.1016/J.BBRC.2017.01.019.
@article{osti_22696855,
title = {microRNA-495 promotes bladder cancer cell growth and invasion by targeting phosphatase and tensin homolog},
author = {Tan, Mingyue and Mu, Xingyu and Liu, Zhihong and Tao, Le and Wang, Jun and Ge, Jifu and Qiu, Jianxin},
abstractNote = {Accumulating evidence has linked deregulation of microRNA-495 (miR-495) to tumorigenesis; however, its function in tumor progression is controversial. This work was undertaken to explore the expression and biological roles of miR-495 in bladder cancer. The expression of miR-495 was examined in 67 pairs of bladder cancer and adjacent normal bladder tissues. The roles of miR-495 in bladder cancer cell proliferation and invasion in vitro and tumorigenesis in vivo were determined. Direct target gene(s) mediating the activity of miR-495 in bladder cancer cells was identified. It was found that miR-495 was expressed at greater levels in bladder tissues and cell lines. High expression of miR-495 was significantly associated with larger tumor size, advanced TNM stage, and lymph node metastasis. Overexpression of miR-495 significantly promoted bladder cancer cell proliferation and invasion, whereas inhibition of miR-495 suppressed cell proliferation and invasion. PTEN, a well-defined tumor suppressor was identified to be a target gene of miR-495. A significant inverse correlation between miR-495 and PTEN expression was noted in bladder cancer tissues (r = −0.3094, P = 0.0125). Overexpression of miR-495 led to reduction of PTEN expression in bladder cancer cells. Rescue experiments showed that enforced expression of PTEN impaired miR-495-mediated bladder cancer proliferation and invasion. In vivo mouse studies demonstrated that overexpression of miR-495 accelerated the growth of subcutaneous bladder cancer xenografts, which was associated with downregulation of PTEN. Overall, these findings indicate that miR-495 upregulation contributes to bladder cancer cell growth, invasion, and tumorigenesis by targeting PTEN and offer a potential therapeutic target for bladder cancer. - Highlights: • miR-495 upregulation induces aggressive phenotype in bladder cancer. • miR-495 is inversely correlated with PTEN in bladder cancer. • miR-495 promotes bladder cancer cell proliferation and invasion by targeting PTEN.},
doi = {10.1016/J.BBRC.2017.01.019},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 483,
place = {United States},
year = {2017},
month = {2}
}