Crystal structure analysis of a hypothetical protein (MJ0366) from Methanocaldococcus jannaschii revealed a novel topological arrangement of the knot fold
Journal Article
·
· Biochemical and Biophysical Research Communications
- Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, 600025 (India)
- RIKEN Structural Biology Laboratory, Yokohama Institute, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, 230-0045 (Japan)
- Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043 (Japan)
The crystal structure of a hypothetical protein MJ0366, derived from Methanocaldococcus jannaschii was solved at 1.9 Å resolution using synchrotron radiation. MJ0366 was crystallized as a monomer and has knot structural arrangement. Intriguingly, the solved structure consists of novel ‘KNOT’ fold conformation. The 3{sub 1} trefoil knot was observed in the structure. The N-terminal and C-terminal ends did not participate in knot formation. - Highlights: • Crystal structure of a hypothetical protein from Methanocaldococcus jannaschii was determined. • The protein adopts novel knot fold conformation. • Structure of the first smallest knot protein was solved.
- OSTI ID:
- 22696752
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 482, Issue 2; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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