microRNA-146a inhibits proliferation, migration and invasion of human cervical and colorectal cancer cells

Journal Article · Fri Nov 25 04:00:00 EST 2016 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2016.10.054· OSTI ID:22696706

Sathyanarayanan, Anusha; Chandrasekaran, Karthik Subramanian; Karunagaran, Devarajan

microRNAs (miRNAs) play significant roles in diverse biological processes and their deregulation is implicated in carcinogenesis. miR-146a executes tumour suppressive or oncogenic functions depending on the cancer type, but its effect on human cervical (CaCx) and colorectal (CRC) cancers have not been examined thus far. In this study, miR-146a exhibited high expression in CaCx but poor expression in CRC, in comparison to corresponding normal tissues. Nevertheless, ectopic expression of miR-146a inhibited proliferation in both CaCx and CRC cells and curbed their migration and invasion. When the expression of endogenous miR-146a was suppressed, proliferative, migratory and invasive capacities of CaCx and CRC cells increased, suggesting an anti-tumourigenic function for miR-146a. Re-expression of miR-146a down-regulated the expression of crucial signalling intermediates: CTNNB1, STAT3, RELA, CCND1 and SNAI1, and enhanced TP53 and CDH1 expression. Thus, the present study reveals a hitherto unknown tumour suppressive role for miR-146a providing a plausible mechanistic basis for it. - Highlights: • miR-146a inhibits proliferation in human cervical and colorectal cancer cells. • miR-146a retards migratory and invasive potentials of cancer cells. • AntimiR-146a enhances growth and motility. • miR-146a modulates the expression of a few genes related to growth and motility. • miR-146a is highly expressed in cervical but poorly so in colorectal tumours.

• OSTI ID: 22696706
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 480; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

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