miR-144-3p serves as a tumor suppressor for renal cell carcinoma and inhibits its invasion and metastasis by targeting MAP3K8
- Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006 (China)
- Department of Breast Surgery, The Fourth Affiliated Hospital of Nanchang University, Nanchang 330003 (China)
MicroRNAs (miRNAs) are important regulators involved in various cancers, including renal cell carcinoma (RCC). The role of the miRNAs involved in RCC progress and metastasis is largely unknown. Here, miRNA microarray analysis was performed to screen the significant miRNAs involved in RCC progress, and miR-144-3p was chosen for further study. We found that the expression of miR-144-3p was significantly lower in RCC specimens and cell lines. In addition, low expression level of miR-144-3p is correlated with tumor progression and poor survival in RCC patients. Based on in vitro assays, we found that miR-144-3p significantly inhibit cancer cell proliferation and progression. Furthermore, function studies revealed that miR-144-3p was significantly correlated with the metastasis potential by affecting the epithelial-mesenchymal transition (EMT). Moreover, Mitogen-activated protein kinase 8 (MAP3K8) is direct target of miR-144-3p, while the expression levels of MAP3K8 were inversely correlated with the expression levels of miR-144-3p in RCC tissues. Overall, our findings demonstrate that miR-144-3p targeted the MAP3K8 pathway to reduce tumor cells proliferation and metastasis in RCC, suggesting that this axis may provide a novel therapeutic target for RCC therapy. - Highlights: • Downregulation of miR-144-3p expression was significantly associated with the poor survival in RCC patients. • Ectopic expression of miR-144-3p inhibits viability and metastasis of RCC cells in vitro. • miR-144-3p suppresses the epithelial-mesenchymal transition in RCC cells. • MAP3K8 is a direct target of miR-144-3p, and miR-144-3p downregulation is a factor in RCC progression through potentiation of MAP3K8 expression.
- OSTI ID:
- 22696681
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 480, Issue 1; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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