Cyclophilin B protects SH-SY5Y human neuroblastoma cells against MPP{sup +}-induced neurotoxicity via JNK pathway

Journal Article · Fri Sep 23 04:00:00 EDT 2016 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2016.08.135· OSTI ID:22696617

Oh, Yoojung; Jeong, Kwon; Kim, Kiyoon; Lee, Young-Seok; Jeong, Suyun ^[1]; Kim, Sung Soo; Yoon, Kyung-Sik; Ha, Joohun; Kang, Insug ^[2]; Choe, Wonchae ^[2]

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1. Department of Biomedical Science, Graduate School, Kyung Hee University (Korea, Republic of)
2. Department of Biochemistry and Molecular Biology, Medicine Research Center for Bioreaction of Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University (Korea, Republic of)

Parkinson's disease (PD) is the second most common neurodegenerative disorder of aging. PD involves a progressive loss of dopaminergic neurons in the substantia nigra pars compacta. 1-Methyl-4-phenyl-1, 2, 3, 6-tetrahydropyidine (MPTP) and its toxic metabolite 1-methyl-4-phenylpyridinium ion (MPP+) inhibit the complex I of the mitochondrial electron transport chain, and have been widely used to construct PD models. Cyclophilin B (CypB) is an endoplasmic reticulum protein that binds to cyclosporine A as a cyclophilin family member. CypB has peptidyl-prolyl cis-trans isomerase (PPIase) activity. We investigated the protective effects of overexpressed CypB on MPP+-induced neurocytotoxicity in SH-SY5Y human neuroblastoma cells. Overexpressed CypB decreased MPP{sup +}-induced oxidative stress through the modulation of antioxidant enzymes including manganese superoxide dismutase and catalase, and prevented neurocytotoxicity via mitogen-activated protein kinase, especially the c-Jun N-terminal kinase pathway. In addition, CypB inhibited the activation of MPP{sup +}-induced the pro-apoptotic molecules poly (ADP-ribose) polymerase, Bax, and Bcl-2, and attenuated MPP{sup +}-induced mitochondrial dysfunction. The data suggest that overexpressed CypB protects neuronal cells from MPP+-induced dopaminergic neuronal cell death. - Highlights: • CypB protects SH-SY5Y cells against MPP{sup +}-induced cell death. • CypB suppresses MPP{sup +}-induced oxidative stress. • CypB inhibits the activation of MPP{sup +}-induced pro-apoptotic and MAPK proteins. • CypB attenuates MPP{sup +}-induced mitochondrial dysfunction.

• OSTI ID: 22696617
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 478; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ENDOPLASMIC RETICULUM
INHIBITION
IONS
MITOCHONDRIA
NERVE CELLS
SUPEROXIDE DISMUTASE