Cytoarchitecture of Zika virus infection in human neuroblastoma and Aedes albopictus cell lines

Journal Article · Sun Jan 15 04:00:00 EST 2017 · Virology

DOI:https://doi.org/10.1016/J.VIROL.2016.11.002· OSTI ID:22692238

Offerdahl, Danielle K. ^[1]; Dorward, David W.; Hansen, Bryan T. ^[2]

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1. Laboratory of Virology, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT (United States)
2. Microscopy Unit, Research Technology Branch, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT (United States)

The Zika virus (ZIKV) pandemic is a global concern due to its role in the development of congenital anomalies of the central nervous system. This mosquito-borne flavivirus alternates between mammalian and mosquito hosts, but information about the biogenesis of ZIKV is limited. Using a human neuroblastoma cell line (SK-N-SH) and an Aedes albopictus mosquito cell line (C6/36), we characterized ZIKV infection by immunofluorescence, transmission electron microscopy (TEM), and electron tomography (ET) to better understand infection in these disparate host cells. ZIKV replicated well in both cell lines, but infected SK-N-SH cells suffered a lytic crisis. Flaviviruses scavenge host cell membranes to serve as replication platforms and ZIKV showed the hallmarks of this process. Via TEM, we identified virus particles and 60–100 nm spherular vesicles. ET revealed these vesicular replication compartments contain smaller 20–30 nm spherular structures. Our studies indicate that SK-N-SH and C6/36 cells are relevant models for viral cytoarchitecture study. - Highlights: •First electron tomography of Zika virus cytoarchitecture. •Comparison of Zika virus infection in human neuroblastoma and mosquito cells. •Ultrastructure of Zika virus infection in human neuroblastoma and mosquito cells.

• OSTI ID: 22692238
• Journal Information: Virology, Journal Name: Virology Vol. 501; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
COMPARATIVE EVALUATIONS
COMPARTMENTS
CONGENITAL DISEASES
MOSQUITOES
PARTICLES
TOMOGRAPHY
TRANSMISSION
TRANSMISSION ELECTRON MICROSCOPY
VIRUSES