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Title: Aluminum exposure for one hour decreases vascular reactivity in conductance and resistance arteries in rats

Abstract

Aims: Aluminum (Al) is an important environmental contaminant; however, there are not enough evidences of Al-induced cardiovascular dysfunction. We investigated the effects of acute exposure to aluminum chloride (AlCl{sub 3}) on blood pressure, vascular reactivity and oxidative stress. Methods and results: Male Wistar rats were divided into two groups: Untreated: vehicle (ultrapure water, ip) and AlCl{sub 3}: single dose of AlCl{sub 3} (100 mg/kg,ip). Concentration-response curves to phenylephrine in the absence and presence of endothelium, the nitric oxide synthase inhibitor L-NAME, the potassium channel blocker tetraethylammonium, and the NADPH oxidase inhibitor apocynin were performed in segments from aortic and mesenteric resistance arteries. NO released was assessed in aorta and reactive oxygen species (ROS), malondialdehyde, non-protein thiol levels, antioxidant capacity and enzymatic antioxidant activities were investigated in plasma, aorta and/or mesenteric arteries. After one hour of AlCl{sub 3} exposure serum Al levels attained 147.7 ± 25.0 μg/L. Al treatment: 1) did not affect blood pressure, heart rate and vasodilator responses induced by acetylcholine or sodium nitroprusside; 2) decreased phenylephrine-induced vasoconstrictor responses; 3) increased endothelial modulation of contractile responses, NO release and vascular ROS production from NADPH oxidase; 4) increased plasmatic, aortic and mesenteric malondialdehyde and ROS production, and 5) decreased antioxidantmore » capacity and affected the antioxidant biomarkers non-protein thiol levels, glutathione peroxidase, glutathione-S-transferase, superoxide dismutase and catalase enzymatic activities. Conclusion: AlCl{sub 3}-acute exposure reduces vascular reactivity. This effect is associated with increased NO production, probably acting on K{sup +} channels, which seems to occur as a compensatory mechanism against Al-induced oxidative stress. Our results suggest that Al exerts toxic effects to the vascular system. - Highlights: • One hour of aluminum exposure decreases vascular reactivity. • Vascular imbalance after aluminum exposure is related to increased oxidative stress. • Enhanced NO release is the first step against the effects of Al at vascular system.« less

Authors:
; ; ; ; ;  [1];  [2];  [3];  [1]
  1. Postgraduate Program in Biochemistry, Universidade Federal do Pampa, Uruguaiana, Rio Grande do Sul (Brazil)
  2. Department of Physiological Sciences, Universidade Federal do Espírito Santo, Espirito Santo (Brazil)
  3. Department of Basic Health Sciences, Universidad Rey Juan Carlos, Alcorcón (Spain)
Publication Date:
OSTI Identifier:
22690876
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 313; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACETYLCHOLINE; ACUTE EXPOSURE; ALUMINIUM; ALUMINIUM CHLORIDES; ANTIOXIDANTS; AORTA; BIOLOGICAL MARKERS; BLOOD; BLOOD PRESSURE; CATALASE; CONCENTRATION RATIO; ENDOTHELIUM; GLUTATHIONE; HEART; NITRIC OXIDE; OXIDASES; OXIDATION; POTASSIUM IONS; RATS; REACTIVITY; SUPEROXIDE DISMUTASE; THIOLS; TRANSFERASES; VASOCONSTRICTORS; VASODILATORS

Citation Formats

Schmidt, Patrícia Medeiros, Escobar, Alyne Goulart, Torres, João Guilherme Dini, Martinez, Caroline Silveira, Rizzetti, Danize Aparecida, Kunz, Simone Noremberg, Vassallo, Dalton Valentim, Alonso, María Jesús, Peçanha, Franck Maciel, and Wiggers, Giulia Alessandra, E-mail: giuliawp@gmail.com. Aluminum exposure for one hour decreases vascular reactivity in conductance and resistance arteries in rats. United States: N. p., 2016. Web. doi:10.1016/J.TAAP.2016.10.023.
Schmidt, Patrícia Medeiros, Escobar, Alyne Goulart, Torres, João Guilherme Dini, Martinez, Caroline Silveira, Rizzetti, Danize Aparecida, Kunz, Simone Noremberg, Vassallo, Dalton Valentim, Alonso, María Jesús, Peçanha, Franck Maciel, & Wiggers, Giulia Alessandra, E-mail: giuliawp@gmail.com. Aluminum exposure for one hour decreases vascular reactivity in conductance and resistance arteries in rats. United States. doi:10.1016/J.TAAP.2016.10.023.
Schmidt, Patrícia Medeiros, Escobar, Alyne Goulart, Torres, João Guilherme Dini, Martinez, Caroline Silveira, Rizzetti, Danize Aparecida, Kunz, Simone Noremberg, Vassallo, Dalton Valentim, Alonso, María Jesús, Peçanha, Franck Maciel, and Wiggers, Giulia Alessandra, E-mail: giuliawp@gmail.com. Thu . "Aluminum exposure for one hour decreases vascular reactivity in conductance and resistance arteries in rats". United States. doi:10.1016/J.TAAP.2016.10.023.
@article{osti_22690876,
title = {Aluminum exposure for one hour decreases vascular reactivity in conductance and resistance arteries in rats},
author = {Schmidt, Patrícia Medeiros and Escobar, Alyne Goulart and Torres, João Guilherme Dini and Martinez, Caroline Silveira and Rizzetti, Danize Aparecida and Kunz, Simone Noremberg and Vassallo, Dalton Valentim and Alonso, María Jesús and Peçanha, Franck Maciel and Wiggers, Giulia Alessandra, E-mail: giuliawp@gmail.com},
abstractNote = {Aims: Aluminum (Al) is an important environmental contaminant; however, there are not enough evidences of Al-induced cardiovascular dysfunction. We investigated the effects of acute exposure to aluminum chloride (AlCl{sub 3}) on blood pressure, vascular reactivity and oxidative stress. Methods and results: Male Wistar rats were divided into two groups: Untreated: vehicle (ultrapure water, ip) and AlCl{sub 3}: single dose of AlCl{sub 3} (100 mg/kg,ip). Concentration-response curves to phenylephrine in the absence and presence of endothelium, the nitric oxide synthase inhibitor L-NAME, the potassium channel blocker tetraethylammonium, and the NADPH oxidase inhibitor apocynin were performed in segments from aortic and mesenteric resistance arteries. NO released was assessed in aorta and reactive oxygen species (ROS), malondialdehyde, non-protein thiol levels, antioxidant capacity and enzymatic antioxidant activities were investigated in plasma, aorta and/or mesenteric arteries. After one hour of AlCl{sub 3} exposure serum Al levels attained 147.7 ± 25.0 μg/L. Al treatment: 1) did not affect blood pressure, heart rate and vasodilator responses induced by acetylcholine or sodium nitroprusside; 2) decreased phenylephrine-induced vasoconstrictor responses; 3) increased endothelial modulation of contractile responses, NO release and vascular ROS production from NADPH oxidase; 4) increased plasmatic, aortic and mesenteric malondialdehyde and ROS production, and 5) decreased antioxidant capacity and affected the antioxidant biomarkers non-protein thiol levels, glutathione peroxidase, glutathione-S-transferase, superoxide dismutase and catalase enzymatic activities. Conclusion: AlCl{sub 3}-acute exposure reduces vascular reactivity. This effect is associated with increased NO production, probably acting on K{sup +} channels, which seems to occur as a compensatory mechanism against Al-induced oxidative stress. Our results suggest that Al exerts toxic effects to the vascular system. - Highlights: • One hour of aluminum exposure decreases vascular reactivity. • Vascular imbalance after aluminum exposure is related to increased oxidative stress. • Enhanced NO release is the first step against the effects of Al at vascular system.},
doi = {10.1016/J.TAAP.2016.10.023},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = ,
volume = 313,
place = {United States},
year = {2016},
month = {12}
}