Chemopreventive effect and angiogenic activity of punicalagin isolated from leaves of Lafoensia pacari A. St.-Hil

Journal Article · Tue Nov 01 00:00:00 EDT 2016 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/J.TAAP.2016.08.015· OSTI ID:22690822

Costa Santos, Suzana da ^[1]; Souza Lino, Ruy de ^[2]; Bara, Maria Teresa Freitas; Chaibub, Beatriz Abdallah ^[3]; Chaves, Dwight Assis ^[4]; Ribeiro da Silva, Antônio Jorge ^[5]; Santos Silva, Luana; Melo e Silva, Daniela de; Chen-Chen, Lee ^[6]

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1. Instituto de Química, Universidade Federal de Goiás, 74001970 Goiânia, GO (Brazil)
2. Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, 74605050 Goiânia, GO (Brazil)
3. Faculdade de Farmácia, Universidade Federal de Goiás, 74605170 Goiânia, GO (Brazil)
4. Laboratório de Estudos Experimentais e Biotecnológicos, Pontifícia Universidade Católica de Goiás, 74605010 Goiânia, GO (Brazil)
5. Núcleo de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, 21941590 Rio de Janeiro, RJ (Brazil)
6. Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001970 Goiânia, GO (Brazil)

Punicalagin is the major ellagitannin constituent from leaves of Lafoensia pacari, a Brazilian medicinal plant widely used for the treatment of peptic ulcer and wound healing. Genotoxic, cytotoxic, antigenotoxic, and anticytotoxic effects of punicalagin were assessed using micronucleus (MN) test and comet assay in mice. Due to the extensive use of L. pacari in the wound healing process, we also assessed the angiogenic activity of punicalagin using the chick chorioallantoic membrane (CAM) angiogenic assay. The highest dose of punicalagin (50 mg/kg) showed significant cytotoxic effect by MN test and in the co-treatment with cyclophosphamide (CPA), this cytotoxicity was enhanced. Co-treatment, pre-treatment and post-treatment of punicalagin with CPA led to a significant reduction in the number of DNA breaks and in the frequency of CPA-induced MN, indicating antigenotoxic effect. Using the CAM model, punicalagin exhibited angiogenic activity in all doses mainly at the lowest concentration (12.5 μg/μL). Therefore, these findings indicate an effective chemopreventive role of punicalagin and a high capacity to induce DNA repair. Also, the angiogenic activity presented by punicalagin in this study could contribute for the processes of tissue repairing and wound healing. - Highlights: • The ellagitannin punicalagin was isolated for the first time from L. pacari leaves. • The genotoxic and chemopreventive effects of punicalagin were evaluated in mice. • The highest dose of punicalagin showed significant cytotoxic effect. • Punicalagin exhibited antigenotoxic effects by the co-, pre- and post-treatment. • Punicalagin showed angiogenic activity and could be applied for tissue repairing.

• OSTI ID: 22690822
• Journal Information: Toxicology and Applied Pharmacology, Vol. 310; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
54 ENVIRONMENTAL SCIENCES
ANIMAL TISSUES
CONCENTRATION RATIO
DNA
DNA REPAIR
DOSES
ENDOXAN
FETAL MEMBRANES
HEALING
MEDICINAL PLANTS
MICE
WOUNDS