Contribution to the risk characterization of ciguatoxins: LOAEL estimated from eight ciguatera fish poisoning events in Guadeloupe (French West Indies)
- Université Paris-Est, ANSES-Laboratory for Food Safety, National Reference Laboratory for the Control of Marine biotoxins, 14 rue Pierre et Marie Curie, 94701 Maisons-Alfort (France)
- Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Ctra. Poble Nou km 5.5, Sant Carles de la Rapita (Spain)
- Ministry of Agriculture, Direction de l’Alimentation de l’Agriculture et de la Forêt de Guadeloupe, Abymes (France)
- Ministry of Agriculture, General Directorate for Food, Paris (France)
- U.S. Food and Drug Administration (FDA), Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, 1 Iberville Drive, Dauphin Island, AL 36528 (United States)
From 2010 to 2012, 35 ciguatera fish poisoning (CFP) events involving 87 individuals who consumed locally-caught fish were reported in Guadeloupe (French West Indies). For 12 of these events, the presence of ciguatoxins (CTXs) was indicated in meal remnants and in uncooked fish by the mouse bioassay (MBA). Caribbean ciguatoxins (C-CTXs) were confirmed by liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis. Using a cell-based assay (CBA), and the only available standard Pacific ciguatoxin-1 (P-CTX-1), the lowest toxins level detected in fish samples causing CFP was 0.022 µg P-CTX-1 equivalent (eq.)·kg{sup −1} fish. Epidemiological and consumption data were compiled for most of the individuals afflicted, and complete data for establishing the lowest observable adverse effects level (LOAEL) were obtained from 8 CFP events involving 21 individuals. Based on toxin intakes, the LOAEL was estimated at 4.2 ng P-CTX-1 eq./individual corresponding to 48.4 pg P-CTX-1 eq. kg{sup −1} body weight (bw). Although based on limited data, these results are consistent with the conclusions of the European Food Safety Authority (EFSA) opinion which indicates that a level of 0.01 µg P-CTX-1 eq. kg{sup −1} fish, regardless of source, should not exert effects in sensitive individuals when consuming a single meal. The calculated LOAEL is also consistent with the U.S. Food and Drug Administration guidance levels for CTXs (0.1 µg C-CTX-1 eq. kg{sup −1} and 0.01 µg P-CTX-1 eq. kg{sup −1} fish). - Highlights: • We report on an epidemiological study on Ciguatera events in the French West Indies. • The collection of consumption data allows for the first time the LOAEL determination. • The LOAEL for ciguatoxins was established at 48.4 pg P-CTX-1 eq. kg{sup −1} bw. • LC–MS/MS provided structural confirmation of C-CTX1 in two suspected samples • Neuro-2A CBA is suitable for assessing composite toxicity levels in fish samples.
- OSTI ID:
- 22687716
- Journal Information:
- Environmental Research, Journal Name: Environmental Research Journal Issue: Part B Vol. 143; ISSN ENVRAL; ISSN 0013-9351
- Country of Publication:
- United States
- Language:
- English
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