Correlation between ECM guidance and actin polymerization on osteogenic differentiation of human adipose-derived stem cells

Journal Article · Sat Oct 01 00:00:00 EDT 2016 · Experimental Cell Research

DOI:https://doi.org/10.1016/J.YEXCR.2016.08.020· OSTI ID:22649766

Keller, Vivian; Deiwick, Andrea ^[1]; Pflaum, Michael ^[2]; Schlie-Wolter, Sabrina ^[1]

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1. Laser Zentrum Hannover e.V., Department of Nanotechnology, Hollerithallee 8, D-30419 Hannover (Germany)
2. Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover (Germany)

The correlation between extracellular matrix (ECM) components, cell shape, and stem cell guidance can shed light in understanding and mimicking the functionality of stem cell niches for various applications. This interplay on osteogenic guidance of human adipose-derived stem cells (hASCs) was focus of this study. Proliferation and osteogenic markers like alkaline phosphatase activity and calcium mineralization were slightly increased by the ECM components laminin (LA), collagen I (COL), and fibronectin (FIB); with control medium no differentiation occurred. ECM guided differentiation was rather dependent on osterix than on Runx2 pathway. FIB significantly enhanced cell elongation even in presence of actin polymerization blockers cytochalasin D (CytoD) and ROCK inhibitor Y-27632, which generally caused more rounded cells. Except for the COL surface, both inhibitors increased the extent of osterix, while the Runx2 pathway was more sensitive to the culture condition. Both inhibitors did not affect hASC proliferation. CytoD enabled osteogenic differentiation independently from the ECM, while it was rather blocked via Y-27632 treatment; on FIB the general highest extent of differentiation occurred. Taken together, the ECM effect on hASCs occurs indirectly and selectively via a dominant role of FIB: it sustains osteogenic differentiation in case of a tension-dependent control of actin polymerization. - Highlights: • Interplay of ECM and cell shape guides osteogenic differentiation of hASCs. • ECM components only present a promotive but not stimulative effect. • No direct correlation between ECM-enhanced cell elongation and differentiation. • Suppression of differentiation depends on a specific actin polymerization blocking. • Fibronectin sustains cell elongation and differentiation in case of blocking actin.

• OSTI ID: 22649766
• Journal Information: Experimental Cell Research, Vol. 347, Issue 2; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACTIN
ALKALINE PHOSPHATASE
BONE MARROW
BUFFERS
CALCIUM
CHANNELING
COLLAGEN
DMSO
ERRORS
HUMAN POPULATIONS
INHIBITION
MATRICES
MINERALIZATION
MORPHOLOGY
MYOSIN
OXIDOREDUCTASES
PHOSPHATES
PHOSPHOTRANSFERASES
POLYMERIZATION
PROLIFERATION
ROCKS
SCANNING ELECTRON MICROSCOPY
SHAPE
SIGNALS
SKELETON
STANDARDS
STEM CELLS
SURFACES
TRANSCRIPTION
TRANSCRIPTION FACTORS