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Title: CTRP6 inhibits fibrogenesis in TGF-β1-stimulated human dermal fibroblasts

Abstract

Skin fibrosis is characterized by excessive proliferation of fibroblasts and overproduction of extracellular matrix (ECM). C1q/tumor necrosis factor-related protein 6 (CTRP6), a member of CTRPs, has been involved in the development of cardiac fibrosis. However, the function and detailed regulatory mechanism of CTRP6 in skin fibrosis remain unclear. The aim of this study was to investigate the effect of CTRP6 on the activation of human dermal fibroblasts. Our results showed that CTRP6 was lowly expressed in scar tissues and transforming growth factor-β1 (TGF-β1)-treated dermal fibroblasts. CTRP6 overexpression significantly inhibited the proliferation of dermal fibroblasts, as well as suppressed the expression of ECM in TGF-β1-treated dermal fibroblasts. Furthermore, CTRP6 overexpression markedly inhibited TGF-β1-induced phosphorylation of Smad3 in dermal fibroblasts. In conclusion, the data reported here demonstrate that CTRP6 is able to inhibit the proliferation and ECM expression in human dermal fibroblasts through suppressing the TGF-β1/Smad3 signaling pathway. These findings suggest that CTRP6 may be a potential therapeutic target for the prevention of skin fibrosis. -- Highlights: •CTRP6 expression was decreased in scar tissues and TGF-β1-treated dermal fibroblasts. •CTRP6 inhibits TGF-β1-induced the proliferation of dermal fibroblasts. •CTRP6 inhibits expression of collagen type I and α-SMA. •CTRP6 inhibits the activation of TGF-β1/Smad3 signalingmore » pathway in dermal fibroblasts.« less

Authors:
 [1]; ;  [1];  [2]; ;  [1]
  1. Department of Burn and Plastic Surgery, Shaanxi Provincial People’s Hospital, Xi’an 710068 (China)
  2. Department of Cosmetology Plastic Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710061 (China)
Publication Date:
OSTI Identifier:
22598780
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 475; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CELL PROLIFERATION; COLLAGEN; FIBROBLASTS; FIBROSIS; GROWTH FACTORS; NECROSIS; NEOPLASMS; PHOSPHORYLATION; SKIN

Citation Formats

Fan, Rong-hui, E-mail: fan_ronghuixa@163.com, Zhu, Xiu-mei, Sun, Yao-wen, Peng, Hui-zi, Wu, Hang-li, and Gao, Wen-jie. CTRP6 inhibits fibrogenesis in TGF-β1-stimulated human dermal fibroblasts. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.05.013.
Fan, Rong-hui, E-mail: fan_ronghuixa@163.com, Zhu, Xiu-mei, Sun, Yao-wen, Peng, Hui-zi, Wu, Hang-li, & Gao, Wen-jie. CTRP6 inhibits fibrogenesis in TGF-β1-stimulated human dermal fibroblasts. United States. doi:10.1016/J.BBRC.2016.05.013.
Fan, Rong-hui, E-mail: fan_ronghuixa@163.com, Zhu, Xiu-mei, Sun, Yao-wen, Peng, Hui-zi, Wu, Hang-li, and Gao, Wen-jie. Fri . "CTRP6 inhibits fibrogenesis in TGF-β1-stimulated human dermal fibroblasts". United States. doi:10.1016/J.BBRC.2016.05.013.
@article{osti_22598780,
title = {CTRP6 inhibits fibrogenesis in TGF-β1-stimulated human dermal fibroblasts},
author = {Fan, Rong-hui, E-mail: fan_ronghuixa@163.com and Zhu, Xiu-mei and Sun, Yao-wen and Peng, Hui-zi and Wu, Hang-li and Gao, Wen-jie},
abstractNote = {Skin fibrosis is characterized by excessive proliferation of fibroblasts and overproduction of extracellular matrix (ECM). C1q/tumor necrosis factor-related protein 6 (CTRP6), a member of CTRPs, has been involved in the development of cardiac fibrosis. However, the function and detailed regulatory mechanism of CTRP6 in skin fibrosis remain unclear. The aim of this study was to investigate the effect of CTRP6 on the activation of human dermal fibroblasts. Our results showed that CTRP6 was lowly expressed in scar tissues and transforming growth factor-β1 (TGF-β1)-treated dermal fibroblasts. CTRP6 overexpression significantly inhibited the proliferation of dermal fibroblasts, as well as suppressed the expression of ECM in TGF-β1-treated dermal fibroblasts. Furthermore, CTRP6 overexpression markedly inhibited TGF-β1-induced phosphorylation of Smad3 in dermal fibroblasts. In conclusion, the data reported here demonstrate that CTRP6 is able to inhibit the proliferation and ECM expression in human dermal fibroblasts through suppressing the TGF-β1/Smad3 signaling pathway. These findings suggest that CTRP6 may be a potential therapeutic target for the prevention of skin fibrosis. -- Highlights: •CTRP6 expression was decreased in scar tissues and TGF-β1-treated dermal fibroblasts. •CTRP6 inhibits TGF-β1-induced the proliferation of dermal fibroblasts. •CTRP6 inhibits expression of collagen type I and α-SMA. •CTRP6 inhibits the activation of TGF-β1/Smad3 signaling pathway in dermal fibroblasts.},
doi = {10.1016/J.BBRC.2016.05.013},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 475,
place = {United States},
year = {Fri Jul 08 00:00:00 EDT 2016},
month = {Fri Jul 08 00:00:00 EDT 2016}
}