Cloning and characterization of a novel Gladiolus hybridus AFP family gene (GhAFP-like) related to corm dormancy

Journal Article · Fri Feb 26 00:00:00 EST 2016 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2016.01.146· OSTI ID:22594268

Wu, Jian; Seng, Shanshan ^[1]; Carianopol, Carina ^[2]; Sui, Juanjuan ^[3]; Yang, Qiuyan; Zhang, Fengqin; Jiang, Huiru ^[1]; He, Junna ^[1]; Yi, Mingfang ^[1]

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1. Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193 (China)
2. Department of Biological Sciences, University of Toronto, Toronto, Ontario (Canada)
3. College of Biology, Fuyang Normal College, Fuyang, Anhui (China)

Abscisic acid (ABA) is an important phytohormone controlling seed dormancy. AFPs (ABA INSENSITIVE FIVE BINDING PROTEINS) are reported to be negative regulators of the ABA signaling pathway. The involvement of AFPs in dormant vegetative organs remains poorly understood. Here, we isolated and characterized a novel AFP family member from Gladiolus dormant cormels, GhAFP-like, containing three conserved domains of the AFP family. Quantitative PCR analysis revealed that GhAFP-like was expressed in dormant organs and its expression was down-regulated along with corm storage. GhAFP-like was verified to be a nuclear-localized protein. Overexpressing GhAFP-like in Arabidopsis thaliana not only showed weaker seed dormancy with insensitivity to ABA, but also changed the expression of some ABA related genes. In addition, a primary root elongation assay showed GhAFP-like may involve in auxin signaling response. The results in this study indicate that GhAFP-like acts as a negative regulator in ABA signaling and is related to dormancy. - Highlights: • GhAFP-like is expessed in dormant corm. • Overexpressing GhAFP-like showed early germination and insensitivity to ABA. • Overexpressing GhAFP-like changed ABI5 downstream genes expression.

• OSTI ID: 22594268
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 471, Issue 1; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ABSCISIC ACID
ARABIDOPSIS
AUXINS
CLONING
ELONGATION
GENES
GERMINATION
ORGANS
POLYMERASE CHAIN REACTION
PROTEINS
ROOTS
SEEDS