Human RNA polymerase II associated factor 1 complex promotes tumorigenesis by activating c-MYC transcription in non-small cell lung cancer

Journal Article · Fri Oct 02 04:00:00 EDT 2015 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2015.08.017· OSTI ID:22592743

Zhi, Xiuyi ^[1]; Giroux-Leprieur, Etienne ^[2]; Wislez, Marie ^[2]; Hu, Mu; Zhang, Yi ^[1]; Shi, Huaiyin ^[3]; Du, Kaiqi ^[4]; Wang, Lei ^[5]

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1. Department of Thoracic Surgery, Xuanwu Hospital, Capital Medical University, Beijing, 100053 (China)
2. ER2 GRC UPMC04 Theranoscan, Pierre et Marie Curie University, Tenon Hospital, 4 Rue de La Chine, 75020, Paris (France)
3. Department of Pathology, Chinese PLA General Hospital, Fu-xing Road #28, Beijing, 100853 (China)
4. Department of Cardiothoracic Surgery, Chinese People's Armed Police Force, Zhejiang Corps Hospital, Jiaxing, Zhejiang Province (China)
5. Department of Human Anatomy, Hebei Medical University, Hebei Province (China)

Human RNA polymerase II (RNAPII)-associated factor 1 complex (hPAF1C) plays a crucial role in protein-coding gene transcription. Overexpression of hPAF1C has been implicated in the initiation and progression of various human cancers. However, the molecular pathways involved in tumorigenesis through hPAF1C remain to be elucidated. The current study suggested hPAF1C expression as a prognostic biomarker for early stage non-small cell lung cancer (NSCLC) and patients with low hPAF1C expression levels had significantly better overall survival. Furthermore, the expression of hPAF1C was found to be positively correlated with c-MYC expression in patient tumor samples and in cancer cell lines. Mechanistic studies indicated that hPAF1C could promote lung cancer cell proliferation through regulating c-MYC transcription. These results demonstrated the prognostic value of hPAF1C in early-stage NSCLC and the role of hPAF1C in the transcriptional regulation of c-MYC oncogene during NSCLC tumorigenesis. - Highlights: • hPAF1C expression is a prognostic biomarker for early stage non-small cell lung cancer. • The expression of hPAF1C was positively correlated with c-MYC in tumor samples of patients and in several NSCLC cell lines. • hPAF1C could promote lung cancer cell proliferation through regulating c-MYC transcription.

• OSTI ID: 22592743
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 465; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BIOLOGICAL MARKERS
CELL PROLIFERATION
HUMAN POPULATIONS
LUNGS
NEOPLASMS
ONCOGENES
PATIENTS
PROTEINS
RNA
RNA POLYMERASES
TRANSCRIPTION