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Determining inhibition effects of some aromatic compounds on peroxidase enzyme purified from white and red cabbage

Journal Article · · AIP Conference Proceedings
DOI:https://doi.org/10.1063/1.4945921· OSTI ID:22591193
 [1];  [1];  [1]
  1. Ataturk University, Science Faculty, Department of Chemistry, 25240-Erzurum (Turkey)
Peroxidases (E.C.1.11.1.7) catalyze the one electron oxidation of wide range of substrates. They are used in synthesis reaction, removal of peroxide from industrial wastes, clinical biochemistry and immunoassays. In this study, the white cabbage (Brassica Oleracea var. capitata f. alba) and red cabbage (Brassica oleracea L. var. capitata f. rubra) peroxidase enzymes were purified for investigation of inhibitory effect of some aromatic compounds on these enzymes. IC{sub 50} values and Ki constants were calculated for the molecules of 6-Amino nicotinic hydrazide, 6-Amino-5-bromo nicotinic hydrazide, 2-Amino-5-hydroxy benzohydrazide, 4-Amino-3-hydroxy benzohydrazide on purified enzymes and inhibition type of these molecules were determined. (This research was supported by Ataturk University. Project Number: BAP-2015/98).
OSTI ID:
22591193
Journal Information:
AIP Conference Proceedings, Journal Name: AIP Conference Proceedings Journal Issue: 1 Vol. 1726; ISSN APCPCS; ISSN 0094-243X
Country of Publication:
United States
Language:
English

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