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Title: Isolation and characterization of Nylanderia fulva virus 1, a positive-sense, single-stranded RNA virus infecting the tawny crazy ant, Nylanderia fulva

Abstract

We report the discovery of Nylanderia fulva virus 1 (NfV-1), the first virus identified and characterized from the ant, Nylanderia fulva. The NfV-1 genome (GenBank accession KX024775) is 10,881 nucleotides in length, encoding one large open reading frame (ORF). Helicase, protease, RNA-dependent RNA polymerase, and jelly-roll capsid protein domains were recognized within the polyprotein. Phylogenetic analysis placed NfV-1 in an unclassified clade of viruses. Electron microscopic examination of negatively stained samples revealed particles with icosahedral symmetry with a diameter of 28.7±1.1 nm. The virus was detected by RT-PCR in larval, pupal, worker and queen developmental stages. However, the replicative strand of NfV-1 was only detected in larvae. Vertical transmission did not appear to occur, but horizontal transmission was facile. The inter-colonial field prevalence of NfV-1 was 52±35% with some local infections reaching 100%. NfV-1 was not detected in limited samples of other Nylanderia species or closely related ant species. - Highlights: • A new positive-strand RNA virus was discovered in the ant, Nylanderia fulva. • The Nylanderia fulva virus 1 genome was comprised of 10,881 nucleotides. • NfV-1 was detected in larval, pupal, queen and worker ants, but not eggs. • Replication of NfV-1 appeared to be limited to themore » larval stage.« less

Authors:
 [1]; ;  [1];  [2];  [3];  [4]
  1. Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Drive, Gainesville, FL 32608 (United States)
  2. Wilkes Honors College, Florida Atlantic University, 5353 Parkside Drive, Jupiter, FL 33458 (United States)
  3. Department of Biological Sciences, Towson University, 8000 York Road, Towson, MD 21252 (United States)
  4. Department of Pathology, University of Cambridge, Cambridge CB2 1QP (United Kingdom)
Publication Date:
OSTI Identifier:
22581705
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 496; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTS; EGGS; LARVAE; NUCLEOTIDES; POLYMERASE CHAIN REACTION; PROTEINS; RNA; VIRUSES

Citation Formats

Valles, Steven M., E-mail: steven.valles@ars.usda.gov, Oi, David H., Becnel, James J., Wetterer, James K., LaPolla, John S., and Firth, Andrew E.. Isolation and characterization of Nylanderia fulva virus 1, a positive-sense, single-stranded RNA virus infecting the tawny crazy ant, Nylanderia fulva. United States: N. p., 2016. Web. doi:10.1016/J.VIROL.2016.06.014.
Valles, Steven M., E-mail: steven.valles@ars.usda.gov, Oi, David H., Becnel, James J., Wetterer, James K., LaPolla, John S., & Firth, Andrew E.. Isolation and characterization of Nylanderia fulva virus 1, a positive-sense, single-stranded RNA virus infecting the tawny crazy ant, Nylanderia fulva. United States. doi:10.1016/J.VIROL.2016.06.014.
Valles, Steven M., E-mail: steven.valles@ars.usda.gov, Oi, David H., Becnel, James J., Wetterer, James K., LaPolla, John S., and Firth, Andrew E.. Thu . "Isolation and characterization of Nylanderia fulva virus 1, a positive-sense, single-stranded RNA virus infecting the tawny crazy ant, Nylanderia fulva". United States. doi:10.1016/J.VIROL.2016.06.014.
@article{osti_22581705,
title = {Isolation and characterization of Nylanderia fulva virus 1, a positive-sense, single-stranded RNA virus infecting the tawny crazy ant, Nylanderia fulva},
author = {Valles, Steven M., E-mail: steven.valles@ars.usda.gov and Oi, David H. and Becnel, James J. and Wetterer, James K. and LaPolla, John S. and Firth, Andrew E.},
abstractNote = {We report the discovery of Nylanderia fulva virus 1 (NfV-1), the first virus identified and characterized from the ant, Nylanderia fulva. The NfV-1 genome (GenBank accession KX024775) is 10,881 nucleotides in length, encoding one large open reading frame (ORF). Helicase, protease, RNA-dependent RNA polymerase, and jelly-roll capsid protein domains were recognized within the polyprotein. Phylogenetic analysis placed NfV-1 in an unclassified clade of viruses. Electron microscopic examination of negatively stained samples revealed particles with icosahedral symmetry with a diameter of 28.7±1.1 nm. The virus was detected by RT-PCR in larval, pupal, worker and queen developmental stages. However, the replicative strand of NfV-1 was only detected in larvae. Vertical transmission did not appear to occur, but horizontal transmission was facile. The inter-colonial field prevalence of NfV-1 was 52±35% with some local infections reaching 100%. NfV-1 was not detected in limited samples of other Nylanderia species or closely related ant species. - Highlights: • A new positive-strand RNA virus was discovered in the ant, Nylanderia fulva. • The Nylanderia fulva virus 1 genome was comprised of 10,881 nucleotides. • NfV-1 was detected in larval, pupal, queen and worker ants, but not eggs. • Replication of NfV-1 appeared to be limited to the larval stage.},
doi = {10.1016/J.VIROL.2016.06.014},
journal = {Virology},
number = ,
volume = 496,
place = {United States},
year = {Thu Sep 15 00:00:00 EDT 2016},
month = {Thu Sep 15 00:00:00 EDT 2016}
}