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Title: Localization of latency-associated nuclear antigen (LANA) on mitotic chromosomes

Abstract

In latent infection of Kaposi's sarcoma-associated herpesvirus (KSHV), viral gene expression is extremely limited and copy numbers of viral genomes remain constant. Latency-associated nuclear antigen (LANA) is known to have a role in maintaining viral genome copy numbers in growing cells. Several studies have shown that LANA is localized in particular regions on mitotic chromosomes, such as centromeres/pericentromeres. We independently examined the distinct localization of LANA on mitotic chromosomes during mitosis, using super-resolution laser confocal microscopy and correlative fluorescence microscopy–electron microscopy (FM-EM) analyses. We found that the majority of LANA were not localized at particular regions such as telomeres/peritelomeres, centromeres/pericentromeres, and cohesion sites, but at the bodies of condensed chromosomes. Thus, LANA may undergo various interactions with the host factors on the condensed chromosomes in order to tether the viral genome to mitotic chromosomes and realize faithful viral genome segregation during cell division. - Highlights: • This is the first report showing LANA dots on mitotic chromosomes by fluorescent microscopy followed by electron microscopy. • LANA dots localized randomly on condensed chromosomes other than centromere/pericentromere and telomere/peritelomre. • Cellular mitotic checkpoint should not be always involved in the segregation of KSHV genomes in the latency.

Authors:
;  [1];  [2];  [1]
  1. Division of Virology, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871 (Japan)
  2. Central Instrumentation Laboratory Research Institute for Microbial Diseases (BIKEN), Osaka University, Osaka 565-0871 (Japan)
Publication Date:
OSTI Identifier:
22581702
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 496; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; CENTROMERES; CHROMOSOMES; ELECTRON MICROSCOPY; FLUORESCENCE; GENES; MITOSIS; SARCOMAS; TELOMERES

Citation Formats

Rahayu, Retno, Ohsaki, Eriko, Omori, Hiroko, and Ueda, Keiji, E-mail: kueda@virus.med.osaka-u.ac.jp. Localization of latency-associated nuclear antigen (LANA) on mitotic chromosomes. United States: N. p., 2016. Web. doi:10.1016/J.VIROL.2016.05.020.
Rahayu, Retno, Ohsaki, Eriko, Omori, Hiroko, & Ueda, Keiji, E-mail: kueda@virus.med.osaka-u.ac.jp. Localization of latency-associated nuclear antigen (LANA) on mitotic chromosomes. United States. doi:10.1016/J.VIROL.2016.05.020.
Rahayu, Retno, Ohsaki, Eriko, Omori, Hiroko, and Ueda, Keiji, E-mail: kueda@virus.med.osaka-u.ac.jp. Thu . "Localization of latency-associated nuclear antigen (LANA) on mitotic chromosomes". United States. doi:10.1016/J.VIROL.2016.05.020.
@article{osti_22581702,
title = {Localization of latency-associated nuclear antigen (LANA) on mitotic chromosomes},
author = {Rahayu, Retno and Ohsaki, Eriko and Omori, Hiroko and Ueda, Keiji, E-mail: kueda@virus.med.osaka-u.ac.jp},
abstractNote = {In latent infection of Kaposi's sarcoma-associated herpesvirus (KSHV), viral gene expression is extremely limited and copy numbers of viral genomes remain constant. Latency-associated nuclear antigen (LANA) is known to have a role in maintaining viral genome copy numbers in growing cells. Several studies have shown that LANA is localized in particular regions on mitotic chromosomes, such as centromeres/pericentromeres. We independently examined the distinct localization of LANA on mitotic chromosomes during mitosis, using super-resolution laser confocal microscopy and correlative fluorescence microscopy–electron microscopy (FM-EM) analyses. We found that the majority of LANA were not localized at particular regions such as telomeres/peritelomeres, centromeres/pericentromeres, and cohesion sites, but at the bodies of condensed chromosomes. Thus, LANA may undergo various interactions with the host factors on the condensed chromosomes in order to tether the viral genome to mitotic chromosomes and realize faithful viral genome segregation during cell division. - Highlights: • This is the first report showing LANA dots on mitotic chromosomes by fluorescent microscopy followed by electron microscopy. • LANA dots localized randomly on condensed chromosomes other than centromere/pericentromere and telomere/peritelomre. • Cellular mitotic checkpoint should not be always involved in the segregation of KSHV genomes in the latency.},
doi = {10.1016/J.VIROL.2016.05.020},
journal = {Virology},
number = ,
volume = 496,
place = {United States},
year = {Thu Sep 15 00:00:00 EDT 2016},
month = {Thu Sep 15 00:00:00 EDT 2016}
}