Fusion of blastomeres in mouse embryos under the action of femtosecond laser radiation. Efficiency of blastocyst formation and embryo development

Journal Article · Sun May 31 00:00:00 EDT 2015 · Quantum Electronics (Woodbury, N.Y.)

DOI:https://doi.org/10.1070/QE2015V045N05ABEH015767· OSTI ID:22551264

Osychenko, A A; Zalesskii, A D; Krivokharchenko, A S; Zhakhbazyan, A K; Nadtochenko, V A ^[1]; Ryabova, A V ^[2]

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1. N N Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow (Russian Federation)
2. A M Prokhorov General Physics Institute, Russian Academy of Sciences, Moscow (Russian Federation)

Using the method of femtosecond laser surgery we study the fusion of two-cell mouse embryos under the action of tightly focused femtosecond laser radiation with the fusion efficiency reaching 60%. The detailed statistical analysis of the efficiency of blastomere fusion and development of the embryo up to the blastocyst stage after exposure of the embryos from different mice to a femtosecond pulse is presented. It is shown that the efficiency of blastocyst formation essentially depends on the biological characteristics of the embryo, namely, the strain and age of the donor mouse. The possibility of obtaining hexaploid embryonal cells using the methods of femtosecond laser surgery is demonstrated. (extreme light fields and their applications)

• OSTI ID: 22551264
• Journal Information: Quantum Electronics (Woodbury, N.Y.), Vol. 45, Issue 5; Other Information: Country of input: International Atomic Energy Agency (IAEA); ISSN 1063-7818
• Country of Publication: United States
• Language: English

Related Subjects

71 CLASSICAL AND QUANTUM MECHANICS
GENERAL PHYSICS
EFFICIENCY
EMBRYOS
LASER RADIATION
MICE
PULSES
STRAINS
SURGERY