Nuclear Factor I-C promotes proliferation and differentiation of apical papilla-derived human stem cells in vitro

Journal Article · Sun Mar 15 04:00:00 EDT 2015 · Experimental Cell Research

DOI:https://doi.org/10.1016/J.YEXCR.2015.01.020· OSTI ID:22462274

Zhang, Jing ^[1]; Wang, Zhihua; Jiang, Yong ^[1]; Niu, Zhongying ^[2]; Fu, Lei; Luo, Zhirong ^[1]; Cooper, Paul R.; Smith, Anthony J. ^[3]; He, Wenxi ^[1]

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1. State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, The Fourth Military Medical University, Xi'an (China)
2. Treatment center of oral diseases, The 306th Hospital of People's Liberation Army, Beijing (China)
3. Oral Biology, School of Dentistry, University of Birmingham, B4 6NN (United Kingdom)

The transcription factor Nuclear Factor I-C (NFIC) has been implicated in the regulation of tooth root development, where it may be anticipated to impact on the behavior of stem cells from the apical papilla (SCAPs) and root odontoblast activity. We hypothesized that NFIC may provide an important target for promoting dentin/root regeneration. In the present study, the effects of NFIC on the proliferation and differentiation of SCAPs were investigated. Over-expression of NFIC increased cell proliferation, mineralization nodule formation and alkaline phosphatase (ALP) activity in SCAPs. Furthermore, NFIC up-regulated the mRNA levels of odontogenic-related markers, ALP, osteocalcin and collagen type I as well as dentin sialoprotein protein levels. In contrast, knockdown of NFIC by si-RNA inhibited the mineralization capacity of SCAPs and down-regulated the expression of odontogenic-related markers. In conclusion, the results indicated that upregulation of NFIC activity in SCAPs may promote osteo/odontoblastic differentiation of SCAPs. - Highlights: • NFIC promotes the proliferation of SCAPs in vitro. • NFIC promotes osteo/odontogenic differentiation of SCAPs in vitro. • Knockdown of NFIC inhibits odontogenic differentiation in SCAPs.

• OSTI ID: 22462274
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 2 Vol. 332; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ALKALINE PHOSPHATASE
CELL PROLIFERATION
COLLAGEN
DENTIN
HUMAN POPULATIONS
IN VITRO
MESSENGER-RNA
MINERALIZATION
STEM CELLS
TEETH
TRANSCRIPTION FACTORS