Zinc oxide nanoparticles induce migration and adhesion of monocytes to endothelial cells and accelerate foam cell formation

Journal Article · Tue Jul 01 04:00:00 EDT 2014 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/J.TAAP.2014.04.010· OSTI ID:22439746

Suzuki, Yuka; Tada-Oikawa, Saeko ^[1]; Ichihara, Gaku ^[2]; Yabata, Masayuki; Izuoka, Kiyora ^[1]; Suzuki, Masako; Sakai, Kiyoshi ^[3]; Ichihara, Sahoko ^[1]

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1. Graduate School of Regional Innovation Studies, Mie University, Tsu (Japan)
2. Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya (Japan)
3. Nagoya City Public Health Research Institute, Nagoya (Japan)

Metal oxide nanoparticles are widely used in industry, cosmetics, and biomedicine. However, the effects of exposure to these nanoparticles on the cardiovascular system remain unknown. The present study investigated the effects of nanosized TiO{sub 2} and ZnO particles on the migration and adhesion of monocytes, which are essential processes in atherosclerogenesis, using an in vitro set-up of human umbilical vein endothelial cells (HUVECs) and human monocytic leukemia cells (THP-1). We also examined the effects of exposure to nanosized metal oxide particles on macrophage cholesterol uptake and foam cell formation. The 16-hour exposure to ZnO particles increased the level of monocyte chemotactic protein-1 (MCP-1) and induced the migration of THP-1 monocyte mediated by increased MCP-1. Exposure to ZnO particles also induced adhesion of THP-1 cells to HUVECs. Moreover, exposure to ZnO particles, but not TiO{sub 2} particles, upregulated the expression of membrane scavenger receptors of modified LDL and increased cholesterol uptake in THP-1 monocytes/macrophages. In the present study, we found that exposure to ZnO particles increased macrophage cholesterol uptake, which was mediated by an upregulation of membrane scavenger receptors of modified LDL. These results suggest that nanosized ZnO particles could potentially enhance atherosclerogenesis and accelerate foam cell formation. - Highlights: • Effects of metal oxide nanoparticles on foam cell formation were investigated. • Exposure to ZnO nanoparticles induced migration and adhesion of monocytes. • Exposure to ZnO nanoparticles increased macrophage cholesterol uptake. • Expression of membrane scavenger receptors of modified LDL was also increased. • These effects were not observed after exposure to TiO{sub 2} nanoparticles.

• OSTI ID: 22439746
• Journal Information: Toxicology and Applied Pharmacology, Journal Name: Toxicology and Applied Pharmacology Journal Issue: 1 Vol. 278; ISSN TXAPA9; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADHESION
ARTERIOSCLEROSIS
CHOLESTEROL
CONSUMER PRODUCTS
FOAMS
ICP MASS SPECTROSCOPY
IN VITRO
INDUSTRY
LEUKEMIA
MACROPHAGES
MEMBRANES
METALS
MOLECULES
MONOCYTES
NANOPARTICLES
RECEPTORS
TITANIUM OXIDES
UPTAKE
ZINC OXIDES